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改良ＨＰＬＣ测定人及大鼠血清中阿昔洛韦浓度的分析方法．使用Ｗａｔｅｒｓ高效液相色谱仪，μ－ＢｏｎｄａｐａｋＣ１８柱，流动相为甲醇—水（３９７，Ｖ／Ｖ），流速１．５ｍＬ／ｍｉｎ，检测波长２５４ｎｍ，内标物为扑热息痛，血清样品采用甲醇去蛋白．阿昔洛韦血清浓度在０．０５～１．６ｍｇ／Ｌ范围内与峰高呈良好线性关系，大鼠和人血清中相对回收率分别为９３．３１％及９０．６９％；最低检测浓度均为０．０２５ｍｇ／Ｌ；天内精密度分别为０．８３％～２．００％及１．１０％～２．５８％；天间精密度分别为２．０１％～５．９０％及３．９０％～６．２７％．
Improved HPLC method for the determination of acyclovir in human and rat serum. The mobile phase was methanol-water (397, V / V), the flow rate was 1.5mL / min, the detection wavelength was 254nm, the internal standard was paracetamol, and the serum sample was methanol deproteinized using Waters High Performance Liquid Chromatography on a μ-Bondapak C18 column. . Aciclovir serum concentration in the range of 0.05 ~ 1.6mg / L showed a good linear relationship with the peak height, the relative recoveries of rat and human serum were 93.31% and 90.69%; the lowest detection concentration The intra-day precision were 0.83% -2.00% and 1.10% -2.58% respectively; the intra-day precision were 2.01% -5.90% and 3 .90% ~ 6.27%.