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将布氏田鼠置1到30天的低温(6±1℃)环境下暴露,其体重与体温没有明显变化;静止代谢率(RMR)、非震颤性产热(NST)及血清中三碘甲腺原氨酸(T_3)含量,随冷暴露时间的延长而增加;肝线粒体状态3呼吸及细胞色素C氧比酶活力亦随冷暴露时间的延长而升高。肝线粒体蛋白质含量经冷暴露30天时有明显升高,表明动物在低温适应中肝线粒体蛋白合成增加与呼吸功能增强是个体RMR提高的细胞学机制之一。布氏田鼠褐色脂肪组织(BAT)的重量及其线粒体蛋白含量在冷暴露10天后明显增加.其线粒体细胞色素C氧化酶、琥珀酸氧化酶及α-磷酸甘油氧化酶活力均在急性冷暴露环境中明显升高,到30天时可增长2一4倍,表明BAT是动物适应寒冷环境的主要产热器官。
The body weight and body temperature of Brandt’s voles (Microtus brandti) were exposed to low temperature (6 ± 1 ℃) for 1 to 30 days. The resting metabolic rate (RMR), non-tremor fever (NST) and serum triiodothyronine The contents of adenosine (T_3) increased with the increase of cold exposure time. The mitochondria state 3 respiration and cytochrome C oxygen specific activity increased with the increase of cold exposure time. Liver mitochondrial protein content increased significantly after 30 days of cold exposure, suggesting that increased mitochondrial protein synthesis and enhanced respiratory function may be one of the more important cellular mechanisms of RMR. The weight and its mitochondrial protein content of B. tabularis brown adipose tissue (BAT) increased significantly after 10 days of cold exposure. The mitochondrial cytochrome C oxidase, succinate oxidase and α-phosphoglycerate oxidase activity were significantly increased in the acute cold exposure environment, can be increased by 2 to 4 times by 30 days, indicating that BAT is the main animal adapt to the cold environment Heat producing organs.