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目的掌握江西省人群中霍乱弧菌菌型变迁、毒力基因、耐药情况,为霍乱防治提供科学依据。方法采用血清学分型法对人群分离的霍乱弧菌进行分型,同时以主要毒力基因ctx为引物对分离的霍乱弧菌进行PCR扩增,并采用改良K-B纸片法对部分菌株进行10种抗生素的药物敏感试验。结果 2006-2008年期间,共从人群中分离到霍乱菌株139株(O139群136株,O1群小川型3株),其中从患者中分离到O139群41株、O1群小川型1株;从密切接触者中分离到O139群95株、O1群小川型2株。毒力实验结果显示O139群中除1株外,其余的均扩增出与阳性对照一致的毒力基因(ctx)条带,而O1群小川型均未扩增出毒力基因(ctx)条带,与阴性对照一致。药敏结果显示:O139群和O1群小川型霍乱弧菌均对利福平、丁胺卡那、氟哌酸、环丙沙星、头孢噻肟100%敏感,耐药性在90%以上的药物是四环素、强力霉素。结论江西省2006-2008年间人群中分离到的霍乱弧菌中,O139群与O1群小川型并存,O139群为优势菌;O139群菌株绝大多数为产毒株,小川型均为非产毒株。药敏结果供霍乱治疗和必要时的预防药物选择提供依据。
Objective To grasp the changes of Vibrio cholerae strains, virulence genes and drug resistance in Jiangxi province and provide a scientific basis for the prevention and treatment of cholera. Methods Vibrio cholerae isolated from the population were genotyped by serotyping method. Vibrio cholerae isolated from ctx, a major virulence gene, were amplified by PCR. Ten strains of bacteria were screened by modified KB disk method. Antibiotic drug sensitivity test. Results A total of 139 strains of cholera were isolated from the population (136 strains of O139 and 3 strains of Ogawa-type O1) from 2006 to 2008. Among them, 41 strains of O139 and Ogawa-type strains were isolated from the patients. Among the close contacts, 95 strains of O139 and 2 strains of Ogawa Ogawa were isolated. The results of virulence test showed that except for one strain in O139 group, the other virulence gene (ctx) bands were amplified consistent with the positive control, whereas the ctx gene was not amplified in O1 group Belt, consistent with negative control. The results of drug susceptibility showed that both Vibrio cholerae O139 and O1 group were 100% sensitive to rifampin, amikacin, norfloxacin, ciprofloxacin and cefotaxime with drug resistance more than 90% The drug is tetracycline, doxycycline. Conclusion Among Vibrio cholerae isolated from the population in Jiangxi province during 2006-2008, O139 group and O1 group Ogawa coexist, and O139 group is the predominant bacteria. O139 group is the most producing strain and Ogawa-type is non-toxigenic Strain. Susceptibility results provide a basis for cholera treatment and preventive drug selection where necessary.