应用双色荧光原位杂交检测慢性粒细胞白血病衍生9号染色体缺失的研究

来源 :中国实用内科杂志 | 被引量 : 0次 | 上传用户:wuxiangff
下载到本地 , 更方便阅读
声明 : 本文档内容版权归属内容提供方 , 如果您对本文有版权争议 , 可与客服联系进行内容授权或下架
论文部分内容阅读
目的研究慢性粒细胞白血病(chronicmye logenous leukemia,CML)患者衍生9号染色体[derivative chromosome 9,der(9)]部分序列缺失情况,探讨双色荧光原位杂交(FISH)技术检测der(9)部分缺失的应用价值。方法对2002年3月至2005年12月中国协和医科大学血液学研究所150例CML患者采用不加任何刺激剂的骨髓24h短期培养法制备染色体,R显带进行核型分析。应用bcr-abl双色双融合DNA探针对骨髓间期细胞行荧光原位杂交,检测der(9)部分序列缺失。结果124例CML患者进行了染色体核型分析,其中3例分析失败,121例患者中典型Ph染色体易位97例,变异易位24例,同时伴附加染色体异常19例。150例CML患者均进行了双色荧光原位杂交检测,其中27例患者der(9)部分缺失,发生率为18.00%。9例为典型Ph染色体易位,占典型Ph染色体易位患者的9.27%;12例为变异易位,占变异易位患者的50.00%。变异易位患者中的der(9)缺失发生率明显高于典型易位患者,差异有统计学意义(P<0.01)。结论在CML患者中der(9)部分缺失的发生率约为18.00%,应用双色双融合FISH技术能够简便、快速、灵敏的检测出CML患者der(9)缺失。 Objective To investigate the deletion of partial sequence of derivative chromosome 9 (der) (9) in patients with chronic myeloid leukemia (CML) and to investigate the loss of der (9) part by dual-color fluorescence in situ hybridization The application value. Methods From March 2002 to December 2005, 150 cases of CML patients from Peking Union Medical College and Institute of Hematology, Chinese People’s Liberation Army were used to prepare chromosomes by short-term culture of bone marrow with no stimulant. R banding was performed for karyotype analysis. The bcr-abl double-stranded double-fusion DNA probe was used to detect the der (9) part of the bone marrow stromal cells by fluorescence in situ hybridization. Results A total of 124 cases of CML patients were analyzed by karyotype analysis, of which 3 failed. Among 121 patients, 97 were typical Ph chromosome translocation, 24 were translocation and 19 were chromosomal abnormalities. Two hundred and fifty cases of CML patients were detected by two-color fluorescence in situ hybridization, of which der (9) was partially deleted in 27 patients, the incidence was 18.00%. Nine cases were typical Ph chromosome translocations, accounting for 9.27% ​​of typical Ph chromosome translocations; 12 cases were translocation, accounting for 50.00% of patients with translocation. The incidence of der (9) deletion in patients with translocation was significantly higher than that in patients with typical translocations (P <0.01). Conclusions The incidence of der (9) partial deletion in CML patients is about 18.00%. The loss of der (9) in CML patients can be detected easily, rapidly and sensitively by using double-color double-fusion FISH.
其他文献
This paper reports the synthesis of a perylene-bridged bis(crown ether) through the condensation reaction between primary amine-armed dibenzo-24-crown-8 and 3,4
Low-dielectric-constant poly(acetoxystyrenezhi-co-octavinyl-polyhedral oligomeric silsesquioxane)(PAS-POSS) organicinorganic hybrid nanocomposite was successful
Seryl-Histidine dipeptide(Ser-His) has been previously reported to be capable of cleaving DNAs and carboxyl esters,as well as proteins.The protein cleavage mech
无单放货有善意的,也有恶意的。一般来说,在货主持有船公司提单,对客户了解的情况下,以电放方式通知船公司无单放货不会有什么问题。90年代以前,发生恶意无单放货的现象较少
2002年10月17日,英特尔公司在京发布了两项全新的无线存储和处理技术,这些技术将有助于大幅度提高移动电话的电池使用寿命、性能和存储能力。 此次发布的产品包括全球第一款
2月3日,休斯敦火箭队主场被姚明的红色球衣覆盖.8年的NBA职业生涯,中国篮球“巨人”姚明将青春全部献给了火箭,如今他的11号红色球衣也高悬丰田中心球馆上空正式退役.带着职
期刊
近日,我国第一份《未成年人网络保护条例》即将颁布,颁布之前,我们不妨来看看这份专门针对未成年人网络保护的法律的送审稿.
期刊
障壁砂坝(障壁岛)不仅是重要的人类生活居住地,还是重要的油气聚集单元。川中上三叠统由滨岸向海方向,障壁砂坝与沼泽、潮坪、泻湖和浅海陆棚相伴生,滨岸局部地区发育有小型
文章通过对高职院女生上体育课的兴趣进行调查,发现女生的体质在逐年下滑,而且对体育课的积极性明显不如男生高反映了不可忽视学校体育工作的客观事实,并就如何提高女生对体
近日,宁波动物园发生老虎咬人事件,一男子为逃票,翻墙进入虎园却被老虎咬死,该事件引发了人们对于“规则”的热议。而同期放映的一组与“规则”相关的名为《让生命无憾》国民
期刊