用薄层色谱法分离测定了反应混合物中的 1 ,5-苯并硫氮杂 艹卓-α-氨基 -β-内酰胺 .扫描条件为 :λ1 =2 80 nm,λ2 =3 54nm,狭缝 1 .2 mm× 1 .2 mm,SX=3 .校准曲线的线性范围是 0 .2 75～ 7.70 μg( γ=0 .990 5,n=9) ,最低检出限为 0 .1 4 0μg.方法的变异系数小于 5% ,平均回收率为 1 0 0 .1 5% ,方法简便、快速 The 1, 5-benzothiazepine-α-amino-β-lactam in the reaction mixture was separated by thin layer chromatography and the scanning conditions were: λ1 = 280 nm, λ2 = 354 nm, 1.2 mm × 1.2 mm, SX = 3. The calibration curve has a linear range of 0.275-7.70 μg (γ = 0.990 5, n = 9) with a minimum detection limit of 0.14 0 μg The coefficient of variation of the method is less than 5%, the average recovery rate is 100.0%, the method is simple and fast