论文部分内容阅读
水稻细菌性条斑病菌(Xamthomonas oryzae pv.oryzicola,Xoc)是水稻的重要病原菌之一,其引起的水稻细菌性条斑病对水稻粮食产量造成重大损失。本研究利用水稻细条病菌广西分离株GX01作为出发菌株,采用同源双交换的方法分别构建rpf C和rpf G的缺失突变体,并研究其生物学功能。结果表明,与野生型GX01相比,缺失突变体Δrpf C和Δrpf G的致病力和在非寄主烟草上的过敏反应严重下降,胞外多糖合成、游动性和渗透压耐受能力明显降低,生物膜形成和絮凝能力显著增加,而互补菌株能基本将表型补回至野生型水平,表明rpf C和rpf G是重要的致病相关基因。本实验为进一步研究rpf C/rpf G基因在水稻条斑病菌的调控机理提供了前期资料。
Xamthomonas oryzae pv.oryzicola (Xoc) is one of the important pathogenic bacteria in rice, and the rice bacterial leaf streak caused by it causes heavy losses to rice grain yield. In this study, GX01 was used as the starting strain of Guangxi Bacillus thuringiensis isolates. The deletion mutants of rpf C and rpf G were constructed by homologous double crossover method and the biological functions were studied. The results showed that compared with wild type GX01, the virulence of Δrpf C and Δrpf G and the anaphylactic reaction on non-host tobacco decreased significantly, the extracellular polysaccharide synthesis, mobility and osmotic pressure tolerance were significantly decreased , Biofilm formation and flocculation capacity increased significantly, while the complementary strains can basically make the phenotype back to the wild-type level, indicating that rpf C and rpf G is an important pathogenicity-related genes. This experiment provides the preliminary data for further study on the regulatory mechanism of rpf C / rpf G gene in rice leaf spot pathogen.