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以OguraCMS紫菜苔×萝卜杂种F1(AR ,2n =19)为母本 ,以甘蓝型油菜 (AACC ,2n =38)为父本进行杂交 ,获得了 4棵杂种植株。其中 1株 (PRN 1)的花色为嵌合体 ,该植株上的花多为黄色 ,但是也有乳白色花 ,另外还有 1朵花甚至 1个花瓣上同时具有黄色和白色区域 ,其余 3株 (PRN 2、 3、 4 )都开白花。PRN 4的花药开花前退化 ,其余 3株都可以看到 3～ 6枚花药 ,能够产生部分花粉 ,但是PRN 2的花粉不能被I2 KI溶液染色。PRN 2具有 4个蜜腺 ,PRN 1和PRN 3具有 2个蜜腺 ,PRN 4无可见蜜腺。在低温下PRN 2叶色正常 ,其余 3株幼叶表现不同程度缺绿。PRN 1的染色体数目为 2n =38,染色体平均配对构型为 14 6 7Ⅰ +10 0 7Ⅱ +1 0 6Ⅲ ,其染色体组构成可能是AACR ;PRN 2的染色体数目为 2n =35 ,染色体平均配对构型为 13 89Ⅰ +8 33Ⅱ +1 33Ⅲ +0 11Ⅳ ,PRN 3的染色体数目为2n =33,染色体平均配对构型为 14 0 0Ⅰ +7 82Ⅱ +1 0 0Ⅲ +0 0 9Ⅳ。PRN 4的染色体数目未能确定。与甘蓝型油菜回交后PRN 1～ 3植株各自产生了一定数量的种子 ,而PRN 4则未产生种子。对这些杂种及其后代的遗传及育种意义进行了讨论
Four hybrid plants were obtained by crossing Ogura CMS with radish F1 (AR, 2n = 19) and rape (AACC, 2n = 38) as the male parent. One of them (PRN 1) was chimeric. The flowers on this plant were mostly yellow, but also had milky flowers. In addition, one flower or even one petal had both yellow and white regions, and the other three strains (PRN 2, 3, 4) are open white flowers. The anthers of PRN 4 degenerated before flowering. The remaining 3 plants all showed 3 ~ 6 anthers, which could produce some pollen, but pollen of PRN 2 could not be stained by I2 KI solution. PRN 2 has 4 nectaries, PRN 1 and PRN 3 have 2 nectaries, and PRN 4 has no visible nectaries. The leaf color of PRN 2 was normal at low temperature, and the remaining three young leaves showed greenishness to varying degrees. The chromosome number of PRN 1 was 2n = 38, the average chromosome pairing configuration was 14 6 7 Ⅰ +10 0 7 Ⅱ +1 0 6 Ⅲ, and the chromosome constitution of PRN 1 was probably AACR. The chromosome number of PRN 2 was 2n = 35, The number of chromosomes was 1389Ⅰ +8 33Ⅱ +1 33Ⅲ +0 11Ⅳ. The chromosome number of PRN 3 was 2n = 33, and the average chromosome pairing configuration was 14 0 0Ⅰ +7 82Ⅱ +1 0 0Ⅲ + 0 0 9 Ⅳ. The number of chromosomes in PRN 4 has not been determined. After backcrossing with B. napus, PRN 1-3 plants each produced a certain amount of seeds, while PRN 4 did not produce seeds. The genetic and breeding implications of these hybrids and their offspring are discussed