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目的:探讨细胞块在宫颈细胞疑难病例中的应用价值。方法:宫颈细胞标本应用液基细胞学技术(TCT)制片诊断,疑难病例制备细胞块并行HE染色及p16INK4A、Ki67染色,将单纯TCT(TCT组)与TCT结合细胞块HE和免疫细胞化学诊断(联合组)进行比较。结果:p16INK4A、Ki67在26例反应性不成熟鳞状化生细胞中均无表达,在22例HSIL阳性表达分别为81.8%(18/22)、90.9%(20/22);在30例绝经的萎缩上皮中均不表达,萎缩背景中的19例HSIL/SCC均阳性表达。TCT组和联合组的符合率分别为70.1%和95.9%,差异具有统计学意义(P<0.05)。结论:疑难宫颈TCT病例行细胞块制作、HE染色及p16INK4A和Ki67染色,可提高宫颈癌筛查的准确性。
Objective: To investigate the value of cell mass in the difficult cases of cervical cells. Methods: Cervical cell samples were diagnosed by liquid-based cytology (TCT). HE staining and p16INK4A, Ki67 staining were used to prepare cell masses in difficult cases. HE staining and immunocytochemistry (Joint group) for comparison. Results: The positive rate of p16INK4A and Ki67 in 26 cases of reactive immature squamous metaplasia was 81.8% (18/22) and 90.9% (20/22), respectively. In 30 cases of menopause Of the atrophic epithelium were not expressed in the atrophic background in 19 cases of HSIL / SCC were positive expression. The coincidence rates of TCT group and combination group were 70.1% and 95.9%, respectively, with statistical significance (P <0.05). Conclusion: Cervical cancer can be diagnosed by cervical intraepithelial neoplasia (TCT), HE staining, p16INK4A and Ki67 staining.