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目的考察非离子表面活性剂维生素E聚乙二醇1000琥珀酸酯(TPGS)对宝藿苷I在Caco-2细胞模型转运的影响。方法采用Caco-2细胞模型研究不同浓度的TPGS对宝藿苷Ⅰ细胞转运行为的影响,以超高压液相色谱(UPLC)法测定细胞样品溶液中宝藿苷I的浓度,计算表观渗透系数(Papp)。结果在应用TPGS后,宝藿苷Ⅰ从细胞绒毛面供给侧(AP)→基底面外侧(BL)的跨膜转运量明显增高(P<0.05),外排比率显著下降(P<0.05)。当宝藿苷Ⅰ与TPGS比例分别为1∶1、1∶3、1∶9时,宝藿苷Ⅰ的外排比率分别为1.978 8、1.779 8、1.609 0,与仅用宝藿苷Ⅰ相比分别下降了73%、76%、78%。结论在Caco-2细胞模型上,TPGS可显著促进宝藿苷Ⅰ的吸收。
Objective To investigate the effect of nonionic surfactant vitamin E polyethylene glycol 1000 succinate (TPGS) on the transport of treasure glycoside I in Caco-2 cells. Methods The Caco-2 cell model was used to study the effect of different concentrations of TPGS on the transit behavior of treasure glycoside I cells. The concentration of treasure glycoside I in the cell sample solution was determined by ultra-high pressure liquid chromatography (UPLC), and the apparent permeability coefficient (Papp). Results After application of TPGS, the translocation of treasure glycoside Ⅰ was significantly increased (P <0.05) from the AP side to the basolateral side (BL) (P <0.05). When the proportion of treasure glycoside Ⅰ and TPGS was 1: 1, 1: 3 and 1: 9 respectively, the efflux rates of treasure glycoside Ⅰ were 1.978 8, 1.779 8 and 1.609 0, respectively, Than the decline of 73%, 76%, 78%. Conclusion TPGS can significantly promote the absorption of treasure glycoside Ⅰ in Caco-2 cell model.