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本文采用液相色谱—质谱联用法(LC/MS),对一种蛋白质—细胞色素C(Cyt.C)的胰蛋白酶酶解产物进行了质量肽谱分析,确定了各肽段在蛋白质中的位置。并用串联质谱(MS/MS)分析了大部分肽段的序列,均获得满意的结果。
In this study, mass spectrometry of tryptic digests of a protein, cytochrome C (Cyt.C), was carried out by liquid chromatography-mass spectrometry (LC / MS) position. The results of tandem mass spectrometry (MS / MS) analysis of most of the peptides showed satisfactory results.