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目的:建立测定降血压肽(AHP)/聚乳酸-羟基乙酸(PLGA)微球中AHP含量和包封率的反相高效液相色谱(RP-HPLC)法。方法:样品经二氯甲烷破坏并用水萃取后进行测定。反相色谱柱为Eclipse XDB-C18,流动相为乙腈-水(含0.05%三氟乙峰酸)均=得1到7∶很83好,流的速分为离1,.0A mHPL.检m测in浓-1,度柱线温性为范30围℃为,2检5～测6波25长μ为g.1m9L9-n(1mr,=进0样.99量9为9),2平0μ均L回。收结率果为:在9此8.7色8%谱,条日件内下和A日H间P与精辅密料度及分溶别为剂0.53%、0.86%(n=5)。结论:所建立的方法操作简单、快速,结果准确、可靠,可用于AHP/PLGA微球中主药含量及包封率的测定。
OBJECTIVE: To establish an RP-HPLC method for the determination of AHP content and entrapment efficiency in blood pressure-reducing peptide (AHP) / polylactic-glycolic acid (PLGA) microspheres. Method: Samples were destructed with methylene chloride and extracted with water. The reversed-phase column was Eclipse XDB-C18 with a mobile phase of acetonitrile-water (0.05% trifluoroacetic acid) = 1 to 7: 83, and the flow rate was divided by 1, .0 mHPL m measured in the concentration of -1, the degree of column temperature of 30 ℃ for the range, 2 check 5 ~ 6 wave measured 25 long μ g.1m9L9-n (1mr, = into 0 like .99 9 9) 2 flat 0μ are L back. The results of the closing rate are as follows: In the 9th and 8.7th color 8% spectra, the intra-day and inter-day H and inter-day doses of 0.5% and 0.86% (n = 5), respectively. Conclusion: The established method is simple, rapid, accurate and reliable, and can be used for determination of the main drug content and entrapment efficiency in the AHP / PLGA microspheres.