论文部分内容阅读
目的应用树突状细胞(DC)在体外诱导高效而特异抗肝癌免疫.方法自肝癌患者外周血中分离DC;以粒/巨噬细胞集落刺激因子(GMCSF)及白介素4(IL4)联合刺激DC;以人肝癌细胞系HepG2肿瘤细胞的肿瘤相关抗原(TAA)激活DC;DC诱导自体T淋巴细胞增殖、分化为细胞毒性T细胞(CTL);检测CTL及其上清液对HepG2肿瘤细胞、LOVO肿瘤细胞及HOS8603肿瘤细胞的细胞毒作用.结果经人肝癌细胞系HepG2肿瘤细胞的TAA激活并经GMCSF及IL4联合刺激后,肝癌患者外周血DC能够诱导自体T淋巴细胞增殖分化为CTL,该CTL及其上清液对HepG2肿瘤细胞均有高效而特异性的杀伤作用(杀伤率分别为92%±105%和41%±89%).结论肝癌患者外周血DC体外能够诱导高效而特异抗肝癌免疫.提示DC作为一新概念上的抗肿瘤疫苗可能在肿瘤治疗及预防中发挥重要作用.
Objective To use dendritic cells (DC) to induce highly efficient and specific anti-hepatocellular carcinoma immunity in vitro. METHODS: DCs were isolated from peripheral blood of patients with hepatocellular carcinoma; DCs were stimulated with granulocyte/macrophage colony-stimulating factor (GM-CSF) and interleukin4 (IL4); tumor-associated antigens of human hepatocellular carcinoma cell line HepG2 tumor cells ( TAA) activates DC; DC induces proliferation of autologous T lymphocytes and differentiates into cytotoxic T cells (CTL); and cytotoxic effects of CTL and its supernatant on HepG2 tumor cells, LOVO tumor cells and HOS8603 tumor cells are detected. Results After TAA activation of human hepatocellular carcinoma cell line HepG2 tumor cells was stimulated by GMCSF and IL4, the peripheral blood DCs of hepatocellular carcinoma cells could induce the proliferation and differentiation of autologous T lymphocytes into CTLs. The CTLs and their supernatants corresponded to HepG2. The tumor cells had highly efficient and specific killing effect (the killing rate was 92%±105% and 41%±89%, respectively). Conclusion Peripheral blood DCs from patients with hepatocellular carcinoma can induce efficient and specific anti-hepatocellular carcinoma immunity in vitro. It is suggested that DC as a new conceptual anti-tumor vaccine may play an important role in the treatment and prevention of cancer.