论文部分内容阅读
本研究利用具有降糖效应的双乙酰丙酮氧钒确定其在两种肾上皮细胞系LLC-PK1和MDCK中诱导产生活性氧物种的来源。利用四种常用的荧光试剂分别检测了VO(acac)2诱导产生的过氧化氢(H2O2)和超氧阴离子(·O2)的水平并确定了它们在细胞内的主要来源部位。实验结果表明,VO(acac)2在LLC-PK1和MDCK两种肾细胞系中均能显著诱导ROS的生成,并且ROS主要来源于线粒体。本研究结果提示,可通过局部降低线粒体部位ROS的水平来减少钒化合物的毒性损伤,同时不影响钒化合物的活性。
In this study, vanadyl diacetylacetonate with hypoglycemic effect was used to determine the source of reactive oxygen species induced in two renal epithelial cell lines, LLC-PK1 and MDCK. Four commonly used fluorescent reagents were used to detect the levels of hydrogen peroxide (H2O2) and superoxide anion (O2) induced by VO (acac) 2, respectively, and identified their main source of intracellular sites. The experimental results show that VO (acac) 2 can significantly induce the production of ROS in both LLC-PK1 and MDCK cell lines, and the ROS are mainly derived from mitochondria. The results of this study suggest that the toxicity of vanadium compounds can be reduced by locally reducing the level of ROS at the mitochondria without affecting the activity of vanadium compounds.