建立柏艾胶囊中槲皮素的含量测定的反向高效液相色谱法,色谱柱Agilent XDB-C18(4.6 mm×150 mm,5μm),流动相为甲醇-0.2 mol/L磷酸溶液(50:50),流速1.0 ml/min,柱温30℃,检测波长256 nm。槲皮素在0.017136~0.25704μg呈良好的线性关系(r2=0.9996);平均回收率为99.89%,RSD为0.8%(n=6)。本法重复性好、灵敏度高、定量准确,可作为柏艾胶囊质量控制方法之一。 The RP-HPLC method was established for the determination of quercetin in Baai Capsules. The column was Agilent XDB-C18 (4.6 mm × 150 mm, 5 μm) with methanol-0.2 mol / L phosphoric acid solution (50: 50), flow rate 1.0 ml / min, column temperature 30 ℃, detection wavelength 256 nm. Quercetin showed a good linearity (r2 = 0.9996) between 0.017136 and 0.25704μg. The average recovery was 99.89% and RSD was 0.8% (n = 6). This method has good repeatability, high sensitivity and accurate quantification, which can be used as one of the quality control methods of Baai Capsules.