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目的 评价万古霉素、奈替米星、阿米卡星、环丙沙星、左氧氟沙星和加替沙星 6种抗菌药物分别与第一代头孢菌素头孢硫脒联合用药 ,对于临床分离的粪肠球菌 (Enterococcusfaecalis,Ef)的体外联合抗菌效应。方法 采用棋盘法设计 ,微量肉汤稀释法测定。测定不同浓度组合的 6组抗菌药物对 30株临床分离的革兰阳性球菌的最低抑菌浓度 ,并计算FIC指数。FIC≤ 0 .5为协同作用 ,0 .5 2为拮抗作用。结果 头孢硫脒对粪肠球菌的MIC50 为 2～ 8mg L ,与万古霉素、奈替米星、阿米卡星、环丙沙星、左氧氟沙星、加替沙星联合应用后 ,其MIC50 分别显著地降低至 0 .1 2 5 ,0 .2 5 ,0 .2 5 ,0 .2 5 ,0 .2 5 ,0 .2 5mg L。FIC指数分布 :FIC≤ 0 .5占 50 %～ 86 .7% ;0 .5 2为 0。结论 6种抗菌药物与头孢硫脒分别联合用药后 ,对粪肠球菌基本表现为协同作用和相加作用 ,并以协同作用为主 ,无关作用较少 ,无拮抗作用
Objective To evaluate the efficacy of vancomycin, netilmicin, amikacin, ciprofloxacin, levofloxacin and gatifloxacin in combination with first-generation cephalosporins cefathiamidine, respectively, for clinical isolates Enterococcus faecalis (Enterococcus faecalis, Ef) in vitro combined antibacterial effect. Methods The method of checkerboard design, trace broth dilution method. To determine the minimum inhibitory concentration (MIC) of 6 antibacterials with different concentrations in 30 clinical isolates of Gram-positive cocci and calculate the FIC index. FIC≤0.5 is synergistic, 0. 5 2 is antagonistic. Results The MIC50 of cefathiamidine to Enterococcus faecalis was 2 ~ 8 mg L, its MIC50 was significantly higher than that of vancomycin, netilmicin, amikacin, ciprofloxacin, levofloxacin and gatifloxacin Reduced to 0 .1 2 5, 0 .2 5, 0 .2 5, 0 .2 5, 0 .2 5, 0 .2 5mg L. FIC index distribution: FIC≤0.5 accounts for 50% -86.7%; 0 .5 2 is zero. Conclusion The combination of 6 antibacterials and cefathiamidine, respectively, showed a synergistic effect and an additive effect on the basic manifestations of Enterococcus faecalis, with a synergistic effect, with little or no antagonism