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目的:探讨细粒棘球绦虫Eg95重组蛋白诱导小鼠的免疫应答效果。方法:设立Eg95重组蛋白免疫组(rEg95组)和对照组(NS组)小鼠分别注射Eg95重组蛋白、福氏佐剂和生理盐水,收集各组血清用酶联免疫吸附(ELISA法)检测IgG水平;采集脾细胞用四甲基偶氮唑盐试验(MTT法)检测免疫小鼠的淋巴细胞增殖反应。结果:Eg95重组蛋白免疫小鼠在第2次免疫后开始检测到抗Eg95抗原的IgG,并随着免疫次数的增多,血清抗体效价升高,在第1次免疫后第10周时,免疫抗体滴度可达到1∶25600。在第4次免疫后,进行淋巴细胞转化实验,MTT法检测证实Eg95重组蛋白免疫的小鼠,其脾细胞可在体外被特异性刺激增生。结论:细粒棘球绦虫Eg95重组蛋白可诱发小鼠产生特异的体液免疫和细胞免疫应答。
Objective: To investigate the immune response induced by Eg95 recombinant Echinococcus granulosus in mice. Methods: Eg95 recombinant protein, Freund’s adjuvant and normal saline were injected into the Eg95 recombinant protein immunized group (rEg95 group) and the control group (NS group) respectively. The serums of each group were collected and detected by enzyme-linked immunosorbent assay (ELISA) The spleen cells were collected and the lymphocyte proliferation reaction of immunized mice was detected by MTT method. Results: Eg95 recombinant protein immunized mice began to detect IgG against Eg95 antigen after the second immunization, and with the increase of immunization times, the serum antibody titer increased. At the 10th week after the first immunization, the immune Antibody titers can reach 1: 25600. After the fourth immunization, lymphocyte transformation was performed. Mice immunized with Eg95 recombinant protein were detected by MTT assay. The splenocytes were stimulated specifically in vitro. Conclusion: Eg95 recombinant Echinococcus granulosus can induce specific humoral and cellular immune response in mice.