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目的:观察百草枯(PQ)急性中毒大鼠所致肺损伤、肝损伤时一氧化氮(NO)和诱导型一氧化氮合酶(iNOS)的变化,探讨生脉注射液对急性百草枯中毒的治疗作用。方法:腹腔注射百草枯引致大鼠中毒并用生脉液进行治疗,观察大体标本、组织病理以及生物学标志:肺湿/干重比、肺泡灌洗液中性粒细胞比和蛋白含量;同时测定肺、肝组织NO含量和iNOS活性。结果:与模型对照组相比,生脉组肺组织病理显示肺淤血、肺水肿、肝出血明显减轻,其生物学标志降低,NO和iNOS也降低;核因子-κB和iNOS也降低。结论:核因子-κB、NO及iNOS对百草枯急性中毒大鼠起重要作用,生脉注射液能降低核因子-κB、NO及iNOS水平,减轻百草枯中毒大鼠肺损伤和肝损伤。
OBJECTIVE: To observe the changes of nitric oxide (NO) and inducible nitric oxide synthase (iNOS) during acute lung injury induced by paraquat (PQ) in rats and to investigate the effect of Shengmai Injection on acute paraquat poisoning. Therapeutic effect. METHODS: Rats were poisoned by intraperitoneal injection of paraquat and treated with Shengmaiye. Gross specimens, histopathology, and biological markers were observed: lung wet/dry weight ratio, neutrophil ratio and protein content in alveolar lavage fluid; simultaneous determination. Lung, liver NO content and iNOS activity. RESULTS: Compared with the model control group, the lung tissue pathology of the Shengmai group showed significantly decreased pulmonary congestion, pulmonary edema, and hepatic hemorrhage. The biological markers were reduced, NO and iNOS were also decreased, and the nuclear factor-κB and iNOS were also decreased. CONCLUSIONS: Nuclear factor-κB, NO, and iNOS play an important role in rats with acute paraquat poisoning. Shengmai injection can reduce the levels of nuclear factor-κB, NO, and iNOS, and reduce lung injury and liver injury in paraquat poisoned rats.