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目的:研究不同促渗剂对莪术软膏中莪术醇、吉马酮透皮吸收的影响。方法:采用Franz扩散池法,以离体大鼠皮肤为透皮屏障,以高效液相色谱法测定加入不同促渗剂(3%冰片、3%薄荷脑、3%冰片+3%薄荷脑)的莪术软膏中莪术醇、吉马酮的累积渗透量,同时测定稳态透皮速率以计算增渗倍数。色谱柱为Diamonsil C18,流动相为乙腈-1%磷酸溶液(梯度洗脱),柱温为25℃,流速为1.0 ml/min,检测波长为216 nm,进样量为20μl。结果:莪术醇、吉马酮检测质量浓度的线性范围分别为9.62~47.11μg/ml(r=0.999 1)、33.61~167.82μg/ml(r=0.999 5);精密度、稳定性试验的RSD均小于2.05%,回收率为99.42%。加入3%冰片、3%薄荷脑、3%冰片+3%薄荷脑后莪术醇、吉马酮10 h平均累积渗透量分别为221.21、209.92、269.44μg/cm2与506.74、480.85、615.67μg/cm2;莪术醇、吉马酮6 h增渗倍数分别为1.62、1.41、1.85与1.66、1.50、2.13。结论:3%冰片+3%薄荷脑对莪术醇和吉马酮促渗效果最佳;本试验为莪术软膏的深入研究提供了一定的理论基础。
Objective: To study the effects of different penetration enhancers on the transdermal absorption of Curcuma longa and Jimamoron in Curcuma ointment. Methods: Franz diffusion cell method was used to establish the transdermal barrier of isolated rat skin. The effects of different penetration enhancers (3% borneol, 3% menthol, 3% borneol and 3% menthol) were determined by high performance liquid chromatography Curcuma ointment Curcuma alcohol, the cumulative infiltration of gemcitabine, while steady-state transdermal rate was measured to calculate the multiplication rate. The column was Diamonsil C18 and the mobile phase was acetonitrile-1% phosphoric acid solution (gradient elution). The column temperature was 25 ℃, the flow rate was 1.0 ml / min, the detection wavelength was 216 nm, and the injection volume was 20μl. Results: The linear ranges of curcumol and gemcitabine were 9.62 ~ 47.11μg / ml (r = 0.999 1) and 33.61 ~ 167.82μg / ml (r = 0.999 5) respectively. The precision and stability test RSD Less than 2.05%, the recovery rate was 99.42%. The average cumulative osmolality of 10% borneol, 3% menthol, 3% borneol, 3% menthol, curcumol and gemcitabine for 10 h were 221.21,209.92,269.44μg / cm2 and 506.74,480.85,615.67μg / cm2 ; Curcumol, gemcitabine 6 h permeation times were 1.62,1.41,1.85 and 1.66,1.50,2.13. CONCLUSION: 3% borneol + 3% menthol has the best effect on curcumol and gemcitabine. This study provides a theoretical basis for the further study of Curcuma ointment.