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The non-covalent interaction between apigenin (API) and different functional monomers (a-methylacrylic acid (MAA), acrylamide (AM), 2-vinylpyridine (2-Vpy) and combined functional monomers (AM/2-Vpy)) was.determined by UV spectrometry, and a series of apigenin molecularly imprinted polymers (API-MIPs) was synthesized with different functional monomers through molecular imprinting technology.The relationship between the non-covalent interaction of template/functional monomer and absorption of MIPs also was studied The results showed that the order of the strength of the non-covalent interaction between AP1 and different functional monomers in tetrahydrofuran (THF) is as follows: 2-Vpy> AM/2-Vpy>AM>MAA, which is positive correlation to the absorption capability of corresponding MIPs, and 2-Vpy is the optimum functional monomer among the used monomer for preparing API- MIPs.