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近年来脑组织移植治疗巴金森氏病使用的是胎儿脑,因排异反应、伦理方面等问题还远远不能适应于临床需要.作者通过自体周围神经节脑内移植来补充脑内多巴胺神经元.本研究通过动物的颈上交感神经节的脑内移植,以探讨治疗巴金森氏病的新方法.方法和结果1.大脑皮质内颈上交感神经的移植用40只大白鼠,全麻下摘出颈上交感神经节,制成2～3块细片,用一细的吸管将颈上交感神经节的细片移植到大脑皮质内,3周后用儿茶酚胺组织荧光法观察移植部位.再用20只大白鼠,移植前7天,在预定移植的大脑皮质内注入6羟基多巴(6-OHDA).移植3周后,移植片中可见许多大型细胞和小型细胞,且从这些细胞伸出许多儿茶酚胺神经纤维,但这些神经纤维几乎未到达脑实质内.移植片在移植前经6羟基多巴处理后,则可见从移植片发出的纤维束伸入脑内.2.甲苯四氢吡啶(MP-TP)致巴金森氏病的颈上交感神经节移植5只日本猴注射MPTP制成巴金森氏病模型,可见纹状
In recent years, brain transplantation for the treatment of Parkinson’s disease is fetal brain, due to rejection, ethical issues are far from the clinical needs of the author through autologous peripheral ganglion intracerebral transplantation to supplement the brain dopamine neurons .In this study, the intra-cranial transplantation of the cervical sympathetic ganglia in animals was used to explore a new method for the treatment of Parkinson’s disease.Methods and Results 1. Transplantation of the cervical sympathetic nerves in the cortex with 40 rats under general anesthesia The cervical sympathetic ganglion was removed and made into 2 to 3 pieces, with a fine straw will be fine cervical sympathetic ganglion transplanted into the cerebral cortex, 3 weeks after catecholamine fluorescence method to observe the site of transplantation. Twenty rats, 6 days prior to transplantation, were injected with 6-hydroxydopamine (6-OHDA) into the intended transplanted cerebral cortex.After 3 weeks of transplantation, many large cells and small cells were seen in the transplanted grafts, Many catecholamine nerve fibers, but these nerve fibers almost did not reach the brain parenchyma. Transplantation in the pre-transplant 6-hydroxy-dopa treatment, we can see that the fibers emitted from the graft into the brain .2 toluene tetrahydropyridine ( MP-TP) to Parkinson’s Sick cervical sympathetic ganglion transplantation 5 Japanese monkey injected MPTP made of Parkinson’s disease model, showing the striated