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探讨小鼠肉瘤 S-180细胞转 IL-2基因后是否存在细胞功能改变。通过 MTT 试验、流式细胞仪分析及电镜观察,结果提示 IL-2基因转染影响 S-180细胞的增殖活性、细胞周期。细胞间连接可能调节细胞通讯并由此抑制瘤组织生长。多种免疫细胞介导的免疫反应及从而导致的凋亡在移植瘤消退中也起重要作用。
To investigate whether there is a change in cell function after transfection of IL-2 gene in mouse sarcoma S-180 cells. By MTT assay, flow cytometry analysis and electron microscopy, the results suggest that IL-2 gene transfection affects the proliferation activity and cell cycle of S-180 cells. Intercellular connections may regulate cellular communication and thereby inhibit tumor tissue growth. A variety of immune cell-mediated immune responses and resulting apoptosis also play an important role in tumor regression.