Antagonistic Effects of Tranilast on Proliferation and Collagen Synthesis Induced by TGF-β_2 in Cul

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Whether tranilast had antagonistic effect on proliferation inhibition and collagen synthesis promotion induced by TGF-β 2 in cultured human trabecular meshwork cells was investigated. Suspension of 1×104 cultured human trabecular meshwork cells of 3—5 passage was distributed in each well of a 96-well disk and divided into control group and experimental group. After 24 h, 0 μg/ml (control), 12.5 μg/ml, 25 μg/ml, 50 μg/ml tranilast with 3.2 ng/ml TGF-β 2 were added into the incubation medium. Another 24 h later, proliferation and collagen synthesis in cultured human trabecular meshwork cells were examined respectively by using tetrazolium-based semiautomated colormetric (MTT) assay and 3H-proline incorporation with liquid scintillation technique. The results showed absorbance (A) values of the experimental groups were 0.9036±0.3017, 1.1361± 0.1352, 1.2457±0.1524 according to the different concentrations of tranilast, and 0.8956± 0.1903 of the control group. In comparison with the control group, 25 μg/ml (q’=3.23, P< 0.05), 50 μg/ml (q’=4.70, P<0.01) tranilast significantly antagonized the decrease of the A values induced by TGF-β 2 in the cultured human trabecular meshwork cells. In comparison with the control group [817.37±124.21 cpm/104 cells], 12.5 μg/ml (620.33±80.46 cpm/104 cells, q’= 4.26, P< 0.05), 25 μg/ml (594.58±88.13 cpm/104 cells, q’=4.81, P<0.01), 50 μg/ml (418.64±67.90 cpm/104 cells, q’=8.62, P<0.01) tranilast significantly inhibited the incorporation of 3H-proline into the cultured human trabecular meshwork cells promoted by TGF-β 2 in a dose-dependent manner. It was concluded that tranilast had the antagonistic effect on the proliferation inhibition and collagen synthesis promotion induced by TGF-β 2 in the cultured human trabecular meshwork cells. Whether tranilast had antagonistic effect on proliferation inhibition and collagen synthesis promoted induced by TGF-β 2 in cultured human trabecular meshwork cells was investigated. Suspension of 1 × 104 cultured human trabecular meshwork cells of 3-5 passages was distributed in each well of a 96 -well disk and divided into control group and experimental group. After 24 h, 0 μg / ml (control), 12.5 μg / ml, 25 μg / ml, 50 μg / ml tranilast with 3.2 ng / ml TGF- into the incubation medium. Another 24 h later, proliferation and collagen synthesis in cultured human trabecular meshwork cells were examined respectively by using tetrazolium-based semiautomated colormetric (MTT) assay and 3H-proline incorporation with liquid scintillation technique. The results showed absorbance (A ) values ​​of the experimental groups were 0.9036 ± 0.3017, 1.1361 ± 0.1352, 1.2457 ± 0.1524 according to the different concentrations of tranilast, and 0.8956 ± 0.1903 of the control group. with the control group 25 μg / ml (q ’= 3.23, P <0.05), 50 μg / ml (q’ = 4.70, P <0.01) the cultured human trabecular meshwork cells. In comparison with the control group [817.37 ± 124.21 cpm / 104 cells], 12.5 μg / ml (620.33 ± 80.46 cpm / 104 cells, q ’= 4.26, (594.58 ± 88.13 cpm / 104 cells, q ’= 4.81, P <0.01), and tranilast significantly inhibited the incorporation of 3H-proline at 418 μg / ml and 418.64 ± 67.90 cpm / 104 cells into the cultured human trabecular meshwork cells promoted by TGF-β 2 in a dose-dependent manner. It was done that tranilast had the antagonistic effect on the proliferation inhibition and collagen synthesis promoted by TGF-β 2 in the cultured human trabecular meshwork cells .
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