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目的探讨重组人腺病毒p53导入不同时限后联合放疗对A549肺腺癌细胞生长抑制、凋亡和p53蛋白表达的影响。方法将外源性p53基因导入人肺腺癌细胞株A549中,加药后即刻、3、6、24和48h联合射线照射(单次4Gy)。MTT检测细胞生长抑制,流式细胞仪检测细胞凋亡,Western blot检测野生型p53蛋白的表达情况。结果加药6、24和48h后放疗组与加药未放疗组、加药即刻放疗组和加药3h后放疗组比较,A549细胞生长抑制率、凋亡率及p53蛋白表达水平均显著增高(P<0.05)。结论为获得对肿瘤细胞的最大杀伤作用,可将放疗时间选定在导入重组人腺病毒p53后6～48h内。
Objective To investigate the effect of recombinant human adenovirus p53 combined with radiotherapy on the growth inhibition, apoptosis and p53 protein expression in A549 lung adenocarcinoma after different time points. Methods The exogenous p53 gene was introduced into human lung adenocarcinoma cell line A549 and irradiated with radiotherapy (single 4Gy) at 3, 6, 24 and 48 h immediately after dosing. Cell growth inhibition was detected by MTT, apoptosis was detected by flow cytometry, and the expression of wild type p53 protein was detected by Western blot. Results After 6, 24 and 48 h, the growth inhibition rate, apoptosis rate and p53 protein expression of A549 cells were significantly increased compared with radiotherapy group, radiotherapy group and radiotherapy group after 3 h treatment P <0.05). Conclusion In order to obtain the maximum killing effect on tumor cells, the radiotherapy time can be selected within 6 ~ 48h after the introduction of recombinant human adenovirus p53.