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目的:探讨癃畅颗粒对体外培养的人前列腺基质细胞增生及凋亡的影响。方法:原代培养增生的人前列腺基质细胞,分别以高、中、低浓度癃畅颗粒处理,MTT法测定细胞增殖指数,TUNEL法检测细胞凋亡。结果:癃畅颗粒处理实验组24 h后前列腺基质细胞的生长活性均被不同程度的抑制,高、中、低浓度组抗增殖指数分别为(0.273±0.0881)%,(0.418±0.0644)%和(0.674±0.0352)%,与空白对照组比较均有显著差异(P<0.05);随着药物浓度的增加,细胞抗增殖指数逐渐升高,各浓度组间比较均有统计学差异(P<0.05);药物处理后细胞凋亡百分率显著增加,高、中、低浓度组凋亡指数分别为(22.12±3.47)%,(44.96±2.81)%和(80.36±10.16)%,与空白对照组比较均有显著差异(P<0.05);随着药物浓度的增加,凋亡指数逐渐升高,各浓度组间比较均有统计学差异(P<0.05)。结论:癃畅颗粒能够显著抑制体外培养的人前列腺基质细胞增殖,促进前列腺基质细胞凋亡。
Objective: To investigate the effects of Cheng Chang granules on proliferation and apoptosis of human prostatic stromal cells cultured in vitro. Methods: Primary cultured human prostate stromal cells were cultured in high, medium and low concentrations of Xuan Chang granules. The proliferation index was measured by MTT assay and the apoptosis was detected by TUNEL method. Results: The growth activity of prostatic stromal cells was inhibited to varying degrees in the experimental group at 24 h, and the anti-proliferation index of high, medium and low concentration groups were (0.273 ± 0.0881)% and (0.418 ± 0.0644)%, respectively (0.674 ± 0.0352)%, respectively. Compared with the blank control group, there was a significant difference (P <0.05). With the increase of the drug concentration, the anti-proliferation index of the cells increased gradually. 0.05). After treatment, the percentage of apoptotic cells increased significantly (22.12 ± 3.47)%, (44.96 ± 2.81)% and (80.36 ± 10.16)%, respectively. Compared with the blank control group (P <0.05). With the increase of drug concentration, the apoptotic index increased gradually. There was a significant difference between each concentration group (P <0.05). Conclusion: Cheongchang Granule can significantly inhibit the proliferation of human prostatic stromal cells in vitro and promote the apoptosis of prostatic stromal cells.