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【目的】扩增日本血吸虫 2 6ku谷胱甘肽硫 转移酶 (Sj2 6GST)基因片段 ,构建其重组原核载体 ,以研制SjGST重组克隆。【方法】根据已知基因序列设计一对引物 ,运用RT PCR技术扩增Sj2 6GST目的基因cDNA片段 ,将其克隆到 pBlue script(KS)质粒中 ,并经酶切、PCR和测序鉴定。【结果】获得GST pBluescript(KS)重组克隆。【结论】本实验获得Sj2 6GST重组克隆 ,为进一步研制基因工程蛋白疫苗作准备。
【Objective】 The objective of this study was to amplify a 26 ku Sj2 6GST gene fragment of Schistosoma japonicum and construct a recombinant prokaryotic vector to develop a recombinant SjGST clone. 【Method】 A pair of primers was designed according to the known gene sequence. The cDNA fragment of Sj2 6GST gene was amplified by RT-PCR and cloned into pBlue script (KS) plasmid. The fragment was identified by restriction enzyme digestion, PCR and sequencing. 【Result】 The recombinant clones of GST pBluescript (KS) were obtained. 【Conclusion】 Sj2 6GST recombinant clones were obtained in this experiment, which can be used to prepare further genetically engineered protein vaccines.