基因治疗是当今肿瘤治疗领域中的热点,尤以药物敏感基因治疗(自杀基因治疗)倍受瞩目,可通过基因修饰手段,将编码外源蛋自的基因转导入肿瘤细胞,使其获得表达,编码某种特定的酶,可将无毒或低毒的前药转化为细胞毒性药物从而选择性杀伤转染目的基因的靶细胞.尿嘧啶磷酸核糖转移酶(UPRT)是存在于细菌等微生物当中的一种酶,哺乳动物当中不存在,可特异性作用于尿嘧啶及其类似物,催化合成嘧啶核苷酸前体UMP,后者是5-FU在体内发挥抗瘤效应最为重要的中间产物,然后才能进一步转化为细胞毒性的代谢产物发挥抗瘤效应.我们采用分子克隆技术成功构 Gene therapy is a hot spot in the field of cancer therapy. Especially, drug sensitive gene therapy (suicide gene therapy) is attracting attention. Genetically modified genes can be used to transduce genes encoding foreign genes into tumor cells. By encoding a specific enzyme, non-toxic or low-toxicity prodrugs can be converted into cytotoxic drugs to selectively kill target cells transfecting the target gene. Uracil phosphoribosyltransferase (UPRT) is present in microorganisms such as bacteria. An enzyme that does not exist in mammals, can specifically act on uracil and its analogs, catalyze the synthesis of pyrimidine nucleotide precursor UMP, which is the most important intermediate for 5-FU to exert its anti-tumor effect in vivo. And then it can be further transformed into cytotoxic metabolites to exert anti-tumor effect. We successfully constructed using molecular cloning technology.