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普通小麦（ＴｒｉｔｉｃｕｍａｅｓｔｉｖｕｍＬ．２ｎ＝４２）“济南１７７”与紫外线照射的高冰草（长穗偃麦草Ａｇｒｏｐｙｒｏｎｅｌｏｎｇａｔｕｍ，２ｎ＝７０）原生质体在ＰＥＧ诱导下融合，获杂种再生植株。取杂种子房诱导产生愈伤组织并再生植株，经染色体和同工酶鉴定，它们仍保留杂种性质。其中两株移栽成活并结实，杂种性质也经表型、染色体、同工酶和ＲＡＰＤ分析得到证明。在Ｆ０和Ｆ１代植株根尖细胞中，均发现高频率地存在着染色体断片；从Ｆ２代花粉母细胞减数分裂的染色体数目及行为发现，杂种细胞染色体数目主要分布在１８Ⅱ～２２Ⅱ，染色体断片发生配对及分离，表明它们是小染色体（ｍｉｎｉｃｈｒｏｍｏｓｏｍｅｓ）。Ｆ１及Ｆ２代植株比亲本小麦（“济南１７７”）秆茎粗硬、生长健壮，穗大粒大，已经产生具有优良性状的Ｆ２代穗系
The protoplasts of Agropyronelongatum (2n = 70) irradiated with ultraviolet light and Jinan 177 (Triticum aestivum L.2n = 42) were fused under PEG induction to obtain hybrid plants. The hybrid ovary was induced to produce callus and regenerate plants, which were identified by chromosomes and isozymes, and they retained the hybrid nature. Two of them transplanted alive and strong, the hybrid nature of phenotypic, chromosome, isozyme and RAPD analysis proved. Chromosome fragments were found at high frequency in the apical cells of F0 and F1 plants. The chromosome number and behavior of meiosis in F2 progeny showed that the number of chromosomes in hybrid cells mainly distributed in 18Ⅱ-22Ⅱ, Matching and dissociation occurred, indicating that they are minichromosomes. F1 and F2 plants had stronger stems and harder stems than those of their parents (Jinan 177)