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乙型肝炎病毒(HBV)DNA可整合于人类肝细胞DNA。作者研究了人类肝细胞癌中这些整合体的结构,并把它们分成初级整合体与次级重排产物。通过对初级整合体的结构分析,推断病毒基因组的复制中间体是整合的主要结构。 整合的HBV DNA与靶细胞DNA一定与缺失关联,可能反映整合反应的基质以及机理。靶细胞DNA序列及染色体中整合的靶位,似乎随选择的,表明HBV DNA整合作用可引起随机突变效应。从肝细胞癌
Hepatitis B virus (HBV) DNA can be integrated into human hepatocyte DNA. The authors studied the structure of these integrins in human hepatocellular carcinoma and divided them into primary integrons and secondary rearrangements. Based on the structural analysis of primary integrons, it is concluded that the replication intermediates of the viral genome are the main structures of integration. The integrated HBV DNA and the target cell DNA must be associated with deletion, probably reflecting the matrix and mechanism of the integration reaction. The target DNA sequence and chromosomal integration sites seem to be selective, suggesting that HBV DNA integration can cause random mutation effects. From hepatocellular carcinoma