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[目的]探讨菱角提取物对人肝癌SMMC-7721细胞的增殖抑制及诱导凋亡作用。[方法]采用MTT法检测菱角提取物对SMMC-7721细胞的增殖抑制作用;丫啶橙染色、DNA琼脂糖凝胶电泳和流式细胞仪检测细胞凋亡。[结果]菱角提取物能抑制SMMC-7721细胞的增殖,且呈明显的剂量依赖性。荧光显微镜下细胞呈典型的凋亡形态学改变。[结论]菱角提取物可抑制SMMC-7721细胞的增殖并诱导其凋亡。
[Objective] To investigate the inhibitory effect of water chestnut extract on the proliferation of human hepatoma SMMC-7721 cells and the induction of apoptosis. [Method] The inhibitory effect of water chestnut extract on SMMC-7721 cells was detected by MTT assay. Apoptosis was detected by acridine orange staining, DNA agarose gel electrophoresis and flow cytometry. [Result] Water chestnut extract could inhibit the proliferation of SMMC-7721 cells in a dose-dependent manner. Under fluorescence microscope, the cells showed typical apoptotic morphological changes. [Conclusion] Water chestnut extract can inhibit the proliferation and induce the apoptosis of SMMC-7721 cells.