论文部分内容阅读
目的:观察伊桐水提物的抗氧化作用及其对创伤性脑损伤(TBI)的保护机制。方法:60只SD大鼠随机分为正常对照组、模型对照组、伊桐水提物40g/kg组、伊桐水提物80g/kg组、伊桐水提物160g/kg组、依达拉奉0.005g/kg阳性对照组。应用自由落体击打(Feeney法)造成大鼠创伤性脑损伤模型,伊桐水提物治疗组造模后继续给药14天后取脑,感觉运动功能测试方法评价大鼠创伤性脑损伤(TBI)后神经生理功能的缺陷程度;应用HE染色法观察大鼠大脑皮层的形态学变化。化学比色法测定脑组织中谷胱甘肽过氧化物酶(GSH-Px)活力及丙二醛(MDA)含量,邻苯三酚自氧化法测定超氧阴离子自由基(O2·-)的清除率,邻二氮菲-Fe2+/H2O2体系测定羟基自由基(·OH)的清除率。结果:创伤性脑损伤模型组大鼠与正常对照组相比有明显的神经功能障碍及病理形态学变化,大脑皮层MDA含量显著升高,GSH-Px活性显著降低。神经功能评分显示伊桐水提物160g/kg组与模型组相比分值明显增高,HE染色显示伊桐水提物160g/kg、80g/kg组的病理改变与模型组相比明显减轻;伊桐水提物160g/kg、80g/kg组的MDA含量与模型组相比显著降低,伊桐水提物160g/kg组的GSH-Px活性与模型组相比显著增高。伊桐水提物在体外条件下能明显抑制O2·-和·OH的生成。结论:伊桐水提物能明显改善神经生理功能损伤,在体内外均具有显著的抗氧化活性,对脑组织有显著的保护作用。
OBJECTIVE: To observe the anti-oxidative effects of Ipomoea aquatica extract and its protective mechanism on traumatic brain injury (TBI). Methods: Sixty SD rats were randomly divided into normal control group, model control group, Ipomoea aquatica 40g / kg group, Ipomoea aquatica 80g / kg group, Ipomoea aquatica 160g / kg group, Flavored 0.005g / kg positive control group. The injury model of traumatic brain injury was induced by Feeney’s method. The rats of Ictalurus punctatus aqueous extract treatment group were sacrificed 14 days after the model was established, and the traumatic brain injury (TBI) was evaluated by brain and sensory motor function test ) Neurophysiological defects; HE staining was used to observe the morphological changes of rat cerebral cortex. The activity of glutathione peroxidase (GSH-Px) and the content of malondialdehyde (MDA) in brain tissue were measured by chemical colorimetry, and the scavenging of superoxide anion radical (O2 · -) by pyrogallol autoxidation Rate, phenanthroline-Fe2 + / H2O2 system of hydroxyl radical (· OH) clearance rate. Results: Compared with the normal control group, the traumatic brain injury model group had obvious neurological dysfunction and pathological changes. The content of MDA in cerebral cortex increased significantly and the activity of GSH-Px decreased significantly. The score of neurological function showed that the score of 160g / kg group was significantly higher than that of the model group. HE staining showed that the pathological changes of 160g / kg and 80g / kg group were significantly reduced compared with the model group; Compared with the model group, the content of MDA of 160g / kg and 80g / kg group of Ipomoea aquatica extract significantly decreased, while the activity of GSH-Px of Ipomoea aquatica 160g / kg group was significantly higher than that of model group. Ito tung water extract in vitro conditions can significantly inhibit the O2 · - and · OH generation. Conclusion: Ictalurus punctatus aqueous extract can significantly improve the neurophysiological injury, both in vivo and in vitro have significant antioxidant activity, a significant protective effect on brain tissue.