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用转座子 Tn5对胡萝卜软腐欧氏杆菌胡萝卜亚种 ( Erwinia carotovora subsp.carotovora,Ecc)进行诱变 ,获得 9个游动性改变了的突变体。 M4 32游动性变大 ;M143、M4 51和 M574游动性完全丧失 ;M4 3、M4 9、M330、M72 5和 M72 6游动性较野生型变小。这 9个突变体在大白菜叶柄上的致病力均减弱。游动性变小或丧失的突变体鞭毛数目减少或未发现有鞭毛。
The mutants of Erwinia carotovora subsp. Carotovora (Ecc) were mutagenized with transposon Tn5 to obtain nine mutants with altered motility. The motility of M4 32 became larger; the motility of M143, M4 51 and M574 was completely lost; the mobility of M4 3, M4 9, M330, M72 5 and M72 6 was smaller than that of wild type. The nine mutants showed reduced pathogenicity in Chinese cabbage petiole. The number of mutant flagella with reduced motility or loss of motility decreased or no flagella were found.