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目的比较旋毛虫成囊前期幼虫虫体抗原、排泄分泌抗原和表面抗原对小鼠产生的免疫保护作用。方法分别用旋毛虫成囊前期幼虫虫体抗原、排泄分泌抗原、表面抗原免疫小鼠,同时设佐剂组和阴性对照组,间隔7d免疫1次,共3次。末次免疫后7d,每只小鼠用200条旋毛虫感染期幼虫经口进行攻击感染。感染后7d和30d分别检查各组小鼠肠道成虫数和肌幼虫数;用ELISA测血清中抗旋毛虫肌幼虫IgG抗体。结果虫体抗原、排泄分泌抗原、表面抗原和佐剂组的成虫减虫率分别为84.89%、89.73%、85.65%、2.57%;肌幼虫减虫率分别为71.71%、80.98%、73.66%、5.60%。排泄分泌抗原组、表面抗原组的成虫减虫率(P均<0.05)及肌幼虫减虫率(P均<0.01)均高于虫体抗原组。各免疫组小鼠血清IgG抗体滴度明显升高,虫体抗原组、排泄分泌抗原组和表面抗原组的几何平均倒数滴度分别为2798.89、3474.51、2984.83,分别为阴性对照组(459.32)的6.09、7.56、6.50倍。结论旋毛虫成囊前期幼虫虫体抗原、排泄分泌抗原和表面抗原均能诱导宿主产生较强的抗攻击感染保护力。成囊前期幼虫的排泄分泌抗原显示出更强的免疫原性。
OBJECTIVE: To compare the immunoprotective effects of larval parasites, excretion-secreting antigens and surface antigens of Trichinella spiralis in mice. Methods Trichinella spiralis were used to immunize mice with larvae preeclampsia antigen, excretion and secretion of antigen, surface antigen respectively, and adjuvant group and negative control group were immunized once every 7 days for 3 times. Seven days after the last immunization, each mouse was challenged with 200 Trichinella spiralis infection larvae orally. 7d and 30d after infection, the numbers of intestinal adult worms and muscle larvae in each group were examined. The anti-Trichinella spiralis larvae IgG antibodies in serum were detected by ELISA. Results The adult worms’ worm resistance rates were 84.89%, 89.73%, 85.65% and 2.57% respectively. The worm reduction rates of muscle larvae were 71.71%, 80.98%, 73.66% and 5.60 %. The worm elimination rate (P <0.05) and the larval worm reduction rate (P <0.01) of excretory secretory antigen group and surface antigen group were higher than that of the parasite antigen group. The serum IgG antibody titers in each immunization group were significantly increased. The geometric mean reciprocal titers of parasitic antigen group, excretion-secreted antigen group and surface antigen group were 2798.89,3474.51,2984.83, respectively, which were negative control group (459.32) 6.09, 7.56, 6.50 times. Conclusion Trichinella spiralis larvae preeclampsia parasites, excretion of antigens and surface antigens can induce the host to produce a strong anti-attack infection protection. Excretory secretion of pre-larvae larvae antigen showed more immunogenicity.