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目的:进行喉鳞状上皮细胞癌相关基因的验证研究,为喉鳞状上皮细胞癌相关基因的进一步研究打下基础。方法:在基因芯片筛选结果中挑出部分基因(SENP1、CD109、Lamininα2、Lamininα3),应用RT-PCR、Western blot进一步检测这些基因在12例喉鳞状上皮细胞癌及其癌旁正常组织中mRNA和蛋白水平的表达。结果:RT-PCR结果显示SENP1、CD109、Lamininα3基因在喉鳞状上皮细胞癌组织中表达上调,Lamininα2基因表达下调。Westernblot结果显示SENP1、CD109蛋白在喉鳞状细胞癌组织中的表达明显高于对应的癌旁正常组织。结论:SENP1、CD109、Lamininα2、Lamininα3可能与喉鳞状上皮细胞癌的发生、发展相关,这对从分子水平研究喉癌的发病机制提供了有益的线索。
Objective: To verify the related genes of laryngeal squamous cell carcinoma and lay the foundation for the further study of laryngeal squamous cell carcinoma related genes. Methods: Some genes (SENP1, CD109, Lamininα2, Lamininα3) were picked out by gene chip screening. RT-PCR and Western blot were used to detect the expression of these genes in 12 cases of laryngeal squamous cell carcinoma and its adjacent normal tissues And protein expression. Results: The results of RT-PCR showed that SENP1, CD109 and Lamininα3 were upregulated in Laryngeal squamous cell carcinoma and Laminin α2 was down-regulated. The results of Western blot showed that the expression of SENP1 and CD109 in laryngeal squamous cell carcinoma was significantly higher than that in corresponding normal tissues. CONCLUSIONS: SENP1, CD109, Lamininα2 and Lamininα3 may be related to the occurrence and development of laryngeal squamous cell carcinoma, which provide useful clues for studying the pathogenesis of laryngeal cancer at the molecular level.