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目的研究急性内囊出血后大鼠空肠肠系膜微循环的变化,探讨脑出血并发症的发病机理。方法采用自体尾静脉血注入法建立大鼠急性内囊出血模型,将40只Wistar大鼠随机分为假手术组、内囊出血后3、6、12、24h组,每组8只大鼠。运用微循环显微摄像分析系统,计测各组肠系膜微静脉血流量、切变率、白微栓数,并对切变率和白微栓数进行相关性分析;计测微动脉内轴流和边流的宽度,并计算边流宽度与管径的比值。结果与假手术组比较,急性内囊出血各时间点肠系膜微静脉血流量明显减少(P<0.05),脑出血6、12、24h组切变率减小(P<0.05),而白微栓数增加(P<0.05),切变率与白微栓数呈明显负相关(r=-0.917,P<0.05);脑出血6、12、24h组微动脉内边流宽度与管径的比值较假手术组显著减小(P<0.05)。结论急性内囊出血后大鼠肠系膜微血管血流量减少,切变率降低,白微栓数增多,边流宽度与管径的比值减小,这些微循环障碍可进一步导致靶器官的损伤。
Objective To study the change of mesenteric microcirculation in rats after acute internal capsule hemorrhage and to explore the pathogenesis of complication of cerebral hemorrhage. Methods The model of acute internal capsule hemorrhage was established by autologous tail vein infusion in rats. Forty Wistar rats were randomly divided into sham operation group, 3, 6, 12 and 24 hours after internal capsule hemorrhage, with 8 rats in each group. The microcirculation microscopic imaging analysis system was used to measure the mesenteric venous blood flow, the shear rate and the number of white micro-emboli in each group. The correlation between the rate of change and the number of white micro-emboli was also analyzed. Width, and calculate the ratio of edge width and diameter. Results Compared with the sham-operation group, the mesenteric micro venous flow decreased significantly at each time point after acute ICH (P <0.05), and the shear rate at 6, 12 and 24 hours after ICH decreased (P <0.05) (P <0.05). There was a negative correlation between the shear rate and the number of white micro-embolus (r = -0.917, P <0.05) Significantly reduced (P <0.05). Conclusions The mesenteric capillary blood flow decreased, the shear rate decreased, the number of white micro-emboli increased, and the ratio of lateral flow width to diameter decreased after acute ICH. These micro-circulation disorders could further lead to target organ damage.