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目的探讨2000μW/cm2电磁辐射对大鼠学习记忆功能的影响。方法实验分为空白对照组,假辐射组,1h/d、2h/d、3h/d、4h/d辐射组。将辐射组大鼠固定体位,头部接受功率密度为2000μW/cm2的近场辐射,连续辐射28d。通过Morris水迷宫检测大鼠的学习记忆能力,采用硫堇染色法观察大鼠海马组织结构的变化。结果行为学检测结果表明,假辐射组大鼠各项指标与空白对照组相比均无明显变化(P﹥0.05);而各辐射组大鼠的逃避潜伏期较空白对照组明显延长(P﹤0.05),在空间探索实验中各辐射组大鼠的空间探索次数明显减少(P﹤0.05)。形态学检测结果表明,假辐射组海马神经元形态与数目与空白对照组相比均无明显差异;而辐射组大鼠海马CA1区神经细胞数量显著减少(P﹤0.05),且细胞排列紊乱,胞浆尼氏体明显减少,细胞核固缩。结论 2000μW/cm2电磁辐射可导致大鼠学习记忆功能下降,其机制可能与损伤大鼠海马神经元有关。
Objective To investigate the effect of 2000μW / cm2 electromagnetic radiation on learning and memory in rats. Methods The experiment was divided into blank control group, sham radiation group, 1h / d, 2h / d, 3h / d, 4h / d radiation group. Radiation rats were fixed position, the head to receive power density of 2000μW / cm2 near-field radiation, continuous radiation 28d. The Morris water maze was used to detect the learning and memory ability of rats, and the changes of hippocampus tissue structure were observed by thionine staining. Results The results of behavioral tests showed that there was no significant difference between sham radiation group and blank control group (P> 0.05), while the escape latency of rats in radiation group was significantly longer than that of control group (P <0.05) ). In the space exploration experiment, the number of space exploration in each radiation group was significantly reduced (P <0.05). Morphological test results showed that there was no significant difference in morphology and number of hippocampal neurons between the sham radiation group and the blank control group, while the number of neurons in the hippocampal CA1 area of the radiation group was significantly decreased (P <0.05) Neutropenia was significantly reduced cytoplasm nuclear condensation. Conclusion 2000μW / cm2 electromagnetic radiation can cause learning and memory in rats decreased, the mechanism may be related to the damage of rat hippocampal neurons.