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目的:为了寻找羟基磷灰石(HA)颗粒的蛋白吸附性能与其Zeta电位之间的关系,为研发具有优秀骨修复能力的HA材料提供理论依据。方法:本文采用水热反应法,合成了形状规则的带状HA颗粒,并采用SEM、BET、XRD对其进行表征。检测了HA颗粒在不同p H、PO_4~(3-)浓度中的Zeta电位;选择小牛血清白蛋白(BSA)、溶菌酶(LYS)两种蛋白作为模型蛋白,测试了HA颗粒在不同p H、PO_4~(3-)浓度环境中对BSA或LYS的吸附量。结果:随着吸附体系的p H值逐渐增大,HA颗粒表面负电性迅速增加,HA颗粒对BSA的吸附能力逐渐减弱,对LYS的吸附能力逐渐增强。随着吸附体系中PO_4~(3-)浓度逐渐增大,HA颗粒表面负电性呈先增大后减小的变化趋势,对BSA、LYS的吸附性能均逐渐减弱。结论:p H和PO_4~(3-)浓度的变化,都能引起HA颗粒Zeta电位的变化,前者引起静电作用类型、强度的变化而改变HA蛋白吸附性能;后者引入竞争吸附,蛋白吸附量随竞争离子浓度增多而逐渐减少。
OBJECTIVE: To find out the relationship between the protein adsorption capacity of hydroxyapatite (HA) particles and its Zeta potential and to provide a theoretical basis for the development of HA materials with excellent bone repair ability. Methods: In this paper, HA particles with regular shape were synthesized by hydrothermal method and characterized by SEM, BET and XRD. The Zeta potential of HA particles at different concentrations of p H and PO 4 3- was detected. Two kinds of proteins, bovine serum albumin (BSA) and lysozyme (LYS), were selected as model proteins to test the HA particles’ H, PO_4 ~ (3-) concentration of BSA or LYS adsorption capacity. Results: With the increasing p H value of the adsorption system, the surface electronegativity of HA particles increased rapidly. The adsorption capacity of HA particles to BSA gradually weakened, and the adsorption capacity to LYS gradually increased. With the increasing concentration of PO_4 ~ (3-) in the adsorption system, the surface electronegativity of HA particles first increased and then decreased, and the adsorption properties of BSA and LYS decreased gradually. CONCLUSION: The change of p H and PO 4 4- concentration can cause the change of Zeta potential of HA particles. The former can cause the change of the type and intensity of HA and change the adsorption ability of HA protein. The latter introduces competitive adsorption, With the competitive ion concentration increased and gradually decreased.