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目的 探讨周围神经施万细胞表达 β1,4 半乳糖基转移酶 Ⅰ (β 1,4 GalT Ⅰ )对其增殖的影响。 方法 将构建的正、反义 β 1,4 GalT Ⅰ表达质粒 ,转染到施万细胞中 ,分析转染细胞倍增时间、3H TdR掺入情况以及细胞周期变化。 结果 转染正义β 1,4 GalT Ⅰ后施万细胞的增殖受到抑制 ,这种作用随转染浓度增大而增强 ;在G1 /G0 期的细胞数目增加 ,S期的数目减少。而转染反义 β 1,4 GalT Ⅰ有相反的作用。 结论 β 1,4 GalT Ⅰ对施万细胞的增殖有抑制作用 ,可能在周围神经施万细胞的增殖调节中发挥重要作用。
Objective To investigate the effect of β 1,4-galactosyltransferase Ⅰ (β 1,4 GalT Ⅰ) on the proliferation of Schwann cells. Methods The constructed antisense β 1,4 GalT Ⅰ plasmid was transfected into Schwann cells. The doubling time, 3H TdR incorporation and cell cycle changes were analyzed. Results The proliferation of Schwann cells was inhibited after transfected with sense β 1, 4 GalT Ⅰ. This effect was enhanced with the increase of transfection concentration. The number of cells in G1 / G0 phase increased and the number of S phase decreased. Transfection with antisense β 1,4 GalT I had the opposite effect. Conclusion β 1,4 GalT Ⅰ inhibits the proliferation of Schwann cells and may play an important role in the regulation of proliferation of Schwann cells.