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目的:研究青壮年猝死综合征(sudden manhood death syndrome,SMDS)心脏窦房结组织中G蛋白耦合的内向整流钾(G-coupled inward rectifying K,GIRK1)及L-型钙离子通道蛋白Ca V1.3表达的变化。方法:实验组为2006至2014年徐州医学院病理学教研室尸体解剖中诊断SMDS者14例,对照组为交通事故颅脑损伤死亡者20例,应用免疫组化方法检测GIRK1及Ca V1.3在窦房结组织中的表达。结果:SMDS组窦房结组织中GIRK1的阳性表达率为(20.89±3.17)%,Ca V1.3的阳性表达率为(18.33±1.54)%,均明显低于对照组(P<0.05)。结论:SMDS者中窦房结离子通道GIRK1及Ca V1.3的蛋白表达量显著下调,提示其可能反馈影响迷走神经的心脏调节作用,从而引起呼吸功能的抑制,导致SMDS患者死亡。
Objective: To study G-coupled inward rectifying K (GIRK1) and L-type calcium ion channel protein Ca V1 in sudden cardiac death syndrome (SMDS). 3 expression changes. Methods: The experimental group consisted of 14 patients diagnosed as SMDS in the autopsy department of pathology department of Xuzhou Medical College from 2006 to 2014. The control group was 20 patients died of traumatic brain injury in traffic accident. The expressions of GIRK1 and Ca V1.3 were detected by immunohistochemistry Sinus node tissue expression. Results: The positive expression rate of GIRK1 in sinus node tissue was (20.89 ± 3.17)% in SMDS group and (18.33 ± 1.54)% in Ca V1.3 group, which were significantly lower than that in control group (P <0.05). CONCLUSIONS: The expression of GIRK1 and Ca V1.3 in the ion channels of the sino-atrial node of SMDS patients is significantly downregulated, suggesting that they may affect cardiac regulation of the vagus nerve via feedback and thus lead to the inhibition of respiratory function, resulting in the death of SMDS patients.