自从Kohler和Milstein建立杂交瘤技术以来,在生物学各领域内得到广泛地研究和应用。在内分泌学方面也得到了应用,已经有十几项激素单克隆抗体代替经典抗血清的报道。本文为建立胰岛素单克隆抗体杂交瘤细胞系而进行了研究,现报道如下。 Since the establishment of hybridoma technology by Kohler and Milstein, it has been extensively studied and applied in various fields of biology. In endocrinology has also been applied, there have been more than a dozen hormone monoclonal antibody instead of the classic antiserum was reported. This article to establish insulin monoclonal antibody hybridoma cell lines were studied, are reported below.