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目的通过检测山东东部地区喉鳞状细胞癌中人乳头状瘤病毒(HPV)的DNA及其亚型的表达,探讨HPV感染与喉癌的关系及其在喉癌发病中的意义。方法采用聚合酶链式反应(PCR)扩增和DNA反向点杂交相结合的DNA芯片技术,检测57例喉鳞状细胞瘤各亚型HPV DNA,对照组为同期喉乳头状瘤细胞8例。结果喉癌组HPV感染率为7.02%(4/57),喉乳头状瘤组75.00%(6/8),差异有统计学意义(χ2=24.906,P<0.01);喉癌组高危型HPV16与低危型HPV43感染率分别与喉乳头状瘤比较,差异均无统计学意义(χ2=5.611、0.143,P>0.05);喉乳头状瘤组低危型HPV11与HPV6感染率较喉癌组高,差异均有统计学意义(χ2=8.611、14.702,P<0.05)。结论山东东部地区喉癌患者HPV感染率较低,而低危型HPV6/11与喉乳头状瘤关系较为密切。
Objective To investigate the relationship between HPV infection and laryngeal cancer and its significance in the pathogenesis of laryngeal cancer by detecting the DNA and its subtypes of human papillomavirus (HPV) in laryngeal squamous cell carcinoma in eastern Shandong province. Methods Fifty - seven cases of laryngeal squamous cell carcinoma were detected by polymerase chain reaction (PCR) amplification and DNA reverse dot blot hybridization. The control group consisted of 8 cases of laryngeal papilloma in the same period . Results The HPV infection rate was 7.02% (4/57) in laryngeal cancer group and 75.00% (6/8) in laryngeal papilloma group, the difference was statistically significant (χ2 = 24.906, P <0.01) And low-risk HPV43 infection rate respectively compared with laryngeal papilloma, the difference was not statistically significant (χ2 = 5.611,0.143, P> 0.05); low-risk type HPV laryngeal papilloma HPV6 and HPV6 infection rate than laryngeal cancer group High and difference were statistically significant (χ2 = 8.611,14.702, P <0.05). Conclusion The HPV infection rate of laryngeal cancer patients in eastern Shandong is low, while the low-risk HPV6 / 11 laryngeal papilloma is more closely related.