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采用组织化学、免疫组化和生物化学方法对栉孔扇贝外套膜中的一氧化氮合酶(EC1.14.13.39,NOS)活性进行了研究。组织化学显示结果表明,帆状部上皮细胞呈NOS强阳性,血细胞和神经纤维呈阳性;外套触手内有较多近圆形细胞和大量波浪形神经纤维呈NOS强阳性;边缘膜内有大量神经细胞和神经纤维分别呈NOS强阳性和阳性,外套环走肌束附近和血管内皮周围均有大量近圆形的强阳性细胞分布;中央膜有大量近圆形阳性细胞聚集成团块或分散分布。免疫组化显示表明,血细胞、帆状部和边缘膜上皮细胞呈神经型NOS(nNOS)和内皮型NOS(eNOS)弱阳性,而呈诱导型NOS(iNOS)阳性。生化测定结果表明,总NOS(tNOS)活力和一氧化氮(NO)含量均为中央膜最高,边缘膜次之,外套触手较低,帆状部最低,其中结构型NOS(cNOS)活力也是帆状部的最低,而iNOS活力则是外套触手的最低。栉孔扇贝外套膜可能是其神经系统中NOS的发生中心,NO-NOS体系可在其免疫防御和调节等方面发挥重要的作用。
The activities of nitric oxide synthase (EC1.14.13.39, NOS) in the mantle of scallop Chlamys farreri were studied by histochemistry, immunohistochemistry and biochemical methods. The results of histochemistry showed that the saillike epithelial cells were strongly positive for NOS and positive for blood cells and nerve fibers. There were more nearly round cells and a large number of undulating nerve fibers in the coat tentacles, which were strongly positive for NOS. There were many nerves in the marginal membrane The cells and nerve fibers were strongly positive and positive NOS, respectively. There were a large number of nearly round and strong positive cells near the muscle bundles in the outer and inner circumferences of the outer collar. A large number of nearly round positive cells in the central membrane aggregated into clumps or scattered distribution . Immunohistochemistry showed that the hematopoietic cells, sagittal and marginal membrane epithelial cells were weakly positive for neuronal NOS (nNOS) and endothelial NOS (eNOS), but positive for inducible NOS (iNOS). Biochemical results showed that the total NOS (tNOS) and nitric oxide (NO) contents were highest in the central membrane, followed by the marginal membrane, lower in the tentacle of the coat, and lowest in the sails. The cNOS activity of structural NOS (cNOS) Shaped part of the lowest, and iNOS vitality is the lowest coat tentacles. Zhikong scallop mantle may be the central nervous system NOS, NO-NOS system can play an important role in its immune defense and regulation.