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目的：研究TSH及Graves’TgC对大鼠脂肪细胞的作用。方法：获取大鼠游离的脂肪细胞。溶脱TSH受体。采用A蛋白尾析柱纯化Graves患者的IgG以脂肪细胞内cAMP浓度及释放到孵化液中甘油浓度作为脂肪分解指标，用cAMP及甘油试剂盒测定cAMP浓度及甘油浓度。结果：1mU／ml的TSll能增加大鼠雅高脂肪细胞的cAMP浓度及甘油的释放。脓苷引起cAMP及甘油的TSH剂量依赖曲线向右移。Gare’TgG抑制[125I]-TSH结合于大鼠脂肪细胞膜上ISH受体，并能刺激雅高脂肪细胞内cAMP的形成。结论：ISH受体功能性表达于脂肪细胞，其生物学效应是通过活化腺耷环化酶而实现的。
Objective: To study the effects of TSH and Graves’TgC on rat adipocytes. Methods: Rat free fat cells were obtained. Stroke TSH receptor. The IgG of Graves patients purified with Protein A tailing column was used as lipolysis index for cAMP concentration in adipocytes and glycerol concentration released into hatch solution. The concentrations of cAMP and glycerol were determined by cAMP and glycerol kit. Results: TS11 at 1 mU / ml increased cAMP concentration and release of glycerol in Acc elephantipoie cells. Psosine caused a shift in the TSH dose-dependent curve of cAMP and glycerol to the right. Gare’TgG inhibits the binding of [125I] -TSH to ISH receptors on the rat adipocyte membrane and stimulates the formation of cAMP in Acc elens adipocytes. CONCLUSIONS: ISH receptors are functionally expressed in adipocytes and their biological effects are mediated by activation of adeno-cyclase.