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目的观察中药复方(AAP)对戊四氮(PTZ)致痫小鼠行为及生长变化的影响。方法 60只健康成年雄性昆明小鼠随机分为对照组(CK组)、模型组(PTZ组)、中药大剂量组(AAPl组)、中药中剂量组(AAPm组)、中药小剂量组(AAPs组)和丙戊酸钠组(VPA组),每组10只。对照组和模型组分别给予生理盐水4μL/(g.d))灌胃;中药各组分别给予中药复方大、中、小剂量(14.8mg/g、7.4mg/g、3.7mg/g)灌胃,每天1次;丙戊酸钠组腹腔注射VPA〔20μg/(g.d)〕,连续7d。最后1次灌胃后,除对照组外,其他各组均腹腔内注射PTZ95μg/g,采用Yuhas分级法观察小鼠行为学改变,记录小鼠痫性发作级别、发作潜伏期、发作持续时间;测定各组小鼠体质量增加值。结果与PTZ组相比,AAPs组小鼠发作潜伏期延长,发作持续时间缩短(P<0.05)。与VPA组比较,中药AAPl、AAPm、AAPs各组小鼠体质量增加(P<0.05)。结论 AAP小剂量能延长PTZ致痫小鼠发作潜伏期,缩短PTZ致痫小鼠发作持续时间,抗惊厥效果良好;且AAP能明显增加小鼠体质量。
Objective To observe the effects of Chinese herbal compound (AAP) on the behavior and growth of PTZ-induced epileptic mice. Methods Sixty healthy adult male Kunming mice were randomly divided into control group (CK group), model group (PTZ group), AAP group (AAP group), AAP group (AAP group), low dose group Group) and sodium valproate group (VPA group), 10 in each group. The control group and model group were given normal saline (4μL / (gd)) by intragastric administration. Each group was given intragastric administration of large, medium and small dose of traditional Chinese medicine (14.8mg / g, 7.4mg / g, 3.7mg / Once a day; sodium valproate group intraperitoneal injection of VPA 〔20μg / (gd〕〕 for 7d. After the last gavage, PTZ was intraperitoneally injected with 95μg / g of PTZ, except for the control group. The Yuhas grading method was used to observe the behavioral changes of the mice. The level of seizure, the incubation period and the duration of the attack were recorded. Body weight gain in each group of mice. Results Compared with PTZ group, mice in AAPs group had prolonged seizure latency and shortened seizure duration (P <0.05). Compared with VPA group, the body weight of mice in AAPl, AAPm and AAP groups increased (P <0.05). Conclusions AAP can prolong the incubation period of PTZ-induced epileptic mice and shorten the duration of PTZ-induced epileptic mice, with good anticonvulsant effect. AAP can significantly increase the body weight of mice.