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目的探讨麻醉对大鼠痛觉加工内外侧通路信息处理的影响。方法利用清醒动物神经细胞群单位放电多通道同步记录技术,在大鼠的初级躯体感觉皮层(SI)、丘脑腹后外侧核(VPL)、前扣带皮层(ACC)以及丘脑背内侧核(MD)埋置电极,给予对侧足底伤害性激光刺激,观察大鼠清醒状态下以及戊巴比妥钠麻醉状态下由激光刺激引发的各脑区神经活动的变化。结果与清醒状态相比,戊巴比妥钠麻醉显著降低了伤害性激光刺激所引发的四个脑区神经元活动的增强,同时也抑制了由疼痛引起的内、外侧通路上脑区之间的同步电活动。另外,各脑区的自发放电频率也因麻醉而发生显著改变,包括抑制和增强两种情况。结论麻醉能显著改变疼痛相关的神经活动,表明大脑活动在麻醉与清醒状态下有着很大差别。该结果提示,在研究感觉神经编码尤其是痛觉编码时,最好使用清醒动物记录神经活动,以获得能真正反映生理状态的实验数据。
Objective To investigate the effects of anesthesia on the information processing of the medial and lateral access pathways of pain in rats. Methods Synchronous recording technique of unit discharge in conscious animal neuron population was used to detect the expression of IL-6 in rat primary somatic cortex (SI), ventral thalamic nucleus (VPL), anterior cingulate cortex (ACC) and dorsal medial thalamic nucleus ) Was used to implant the electrodes. The laser injury was induced on the contralateral foot. The changes of neural activity in various brain regions induced by laser stimulation were observed under awake condition in rats and pentobarbital sodium anesthesia. Results Compared with the awake state, sodium pentobarbital anesthesia significantly reduced the increase of neuronal activity in the four brain regions induced by noxious laser stimulation, and also inhibited the intracerebral areas of medial and lateral pathways caused by pain Synchronous electrical activity. In addition, the frequency of spontaneous firing in various brain regions also changed significantly due to anesthesia, both in inhibition and enhancement. Conclusion Anesthesia can significantly alter pain-related neural activity, suggesting that brain activity varies widely between anesthesia and wakefulness. The results suggest that when studying sensory nerve coding, especially pain codes, it is best to use conscious animals to record neural activity to obtain experimental data that truly reflect the physiological state.