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目的:研究鸡腿蘑多糖(CCP)对肝癌细胞体外增生的抑制作用和对小鼠S_(180)腹水瘤与实体瘤的抑制作用。 方法:人肝癌SMMC-7721细胞与CCP共培养后,MTF比色分析法检测活细胞数;显微镜直接计数法测定CCP对小鼠S_(180)腹水瘤细胞有丝分裂指数的影响;腹腔注射CCP,用ANAE法测定CCP对T淋巴细胞增生的作用;观察不同浓度的CCP对肝癌SMMC-7721细胞及小鼠S_(180)腹水瘤、实体瘤生长的影响。 结果:CCP对人肝癌SMMC-7721细胞的体外增生有一定抑制作用,在50-200mg·L~(-1)范围中作用与浓度呈正相关(r=0.8,P<0.01);显著抑制小鼠S_(180)实体瘤和腹水瘤的生长(P<0.05),治疗组50mg·kg~(-1)的CCP对小鼠移植性实体瘤抑瘤率达59％;能明显降低腹水瘤有丝分裂指数(P<0.05),50mg·kg~(-1)的CCP可使小鼠S_(180)腹水瘤有丝分裂指数从3.4％降低到1.5％;50mg·kg~(-1)的CCP使T淋巴细胞数量增长35.38％(P<0.05),通过上调T细胞数量进一步调节细胞免疫,增强机体对肿瘤的免疫能力。 结论:鸡腿蘑多糖(CCP)能抑制肿瘤的生长,具有预防和治疗癌症的潜在价值。
OBJECTIVE: To study the inhibitory effect of Coprinus comatus polysaccharide (CCP) on the proliferation of hepatocellular carcinoma cells in vitro and the inhibition of S 180 ascites tumor and solid tumor in mice. METHODS: Human hepatocellular carcinoma SMMC-7721 cells were co-cultured with CCP, and the number of viable cells was measured by MTF colorimetric assay. The effect of CCP on the mitotic index of S 180 ascites tumor cells was measured by microscope and counted directly; intraperitoneal injection of CCP was used. The effect of CCP on the proliferation of T lymphocytes was measured by ANAE method. The effects of different concentrations of CCP on the growth of hepatoma SMMC-7721 cells and mouse S 180 ascites tumors and solid tumors were observed. RESULTS: CCP inhibited the proliferation of human hepatocellular carcinoma SMMC-7721 cells in vitro, and it was positively correlated with the concentration in the range of 50-200 mg·L -1 (r=0.8, P<0.01). The mice were significantly inhibited. S_(180) growth of solid tumors and ascites tumors (P<0.05). In the treatment group, 50 mg·kg -1 CCP had a tumor inhibition rate of 59% in transplanted solid tumors; it could significantly reduce the mitotic index of ascites tumors. (P<0.05), 50 mg·kg-1(-1) CCP reduced the mitotic index of S180 ascites tumor in mice from 3.4% to 1.5%; 50 mg·kg-1 CCP resulted in T lymphocytes The number increased by 35.38% (P<0.05), and the cellular immunity was further regulated by upregulating the number of T cells to enhance the body’s immunity to tumors. Conclusion: Coprinus comatus polysaccharides (CCP) can inhibit the growth of tumors and have potential value in the prevention and treatment of cancer.