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为探讨脑内胆碱能神经系统与吗啡戒断症状之间关系，作者将体重200～250g的雄性SD大鼠，以戊巴比妥钠麻醉后，按鼠脑图谱在侧脑室埋管，术后休息1周以上，给大鼠皮下递增注射吗啡，每天2次共sd，建立吗啡依赖大鼠模型。模型动物随机分为4组（每组n=8）：①生理盐水组，②东莨碱组，③哌拉唑嗪组，④美索四氨组。在最后一次注射吗啡后2h．将与微量注射器连接的插管，插入各组动物的脑室瘘管内，分别推注25μg东莨碱、160nmolw哌拉唑嗪、140nmol美索四氨、生理盐水（均为10ul）。结果显示：生理盐水组吗啡戒断反应的评价总分明显比其余组高（P＜0．01）。在戒断症状分类指标中，湿犬样颤动、站立、咬牙等次数在东莨碱组、哌拉唑嗪组和美索四氨组均比生理盐水组低．与生理盐水组比，东莨碱能显著对抗吗啡戒断引起的体重丢失（P＜0．01）。上述结果表明：非选择性和选择性胆碱能神经M受体拮抗剂能对抗纳络团引发的戒断症状，胆碱能神经系统对吗啡戒断反应起重要调节作用。
In order to explore the relationship between the intracerebral cholinergic nervous system and morphine withdrawal symptoms, the male SD rats weighing 200-250 g were anesthetized with sodium pentobarbital and then placed in the lateral cerebral ventricle according to the mouse brain atlas. After a rest for more than 1 week, rats were injected subcutaneously with morphine twice a day for a total of SD to establish a morphine-dependent rat model. The model animals were randomly divided into 4 groups (n = 8 in each group): ① saline group, ② scopolamine group, ③ piperazolazine group, ④ methotrexate group. 2h after the last injection of morphine. The cannula connected to the microinjector was inserted into the fistula of the ventricles of each group and were injected with 25 μg of caustic soda, 160 nmolw of piperazolazine, 140 nmol of mesotrione, and normal saline (both 10 ul). The results showed that morphine withdrawal symptoms in normal saline group were significantly higher than those in other groups (P <0.01). In the indicators of withdrawal symptoms, the number of wet dog-like shaking, standing, teething, etc. were lower in the scopolamine, pirazacide and mesotrione groups than in the saline group. Scopolamine was significantly more potent than morphine in losing weight (P <0.01). The above results show that: non-selective and selective cholinergic M receptor antagonists can counteract the withdrawal symptoms caused by the Nalysis group, cholinergic nervous system plays an important role in morphine withdrawal response.