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目的 研究胃癌组织中肝素酶mRNA的表达与胃癌临床病理特征及肿瘤微血管密度之间的关系 ,并探讨胃癌组织中的肝素酶表达增高的机制。方法 采用逆转录 -聚合酶链反应(RT PCR)法检测 4 7例手术切除的胃癌组织及癌旁组织中肝素酶mRNA的表达 ,免疫组织化学法检测c met在胃癌组织中的表达 ,CD34免疫组织化学染色 ,计数肿瘤微血管密度。结果 胃癌组织中肝素酶表达阳性率明显高于相应的癌旁组织 (74 5 %vs 19 1% ,P <0 0 1)。肝素酶表达阳性组的胃癌组织 ,其微血管密度显著高于阴性组 (5 1± 15vs 31± 10 ,P <0 0 1)。肝素酶mRNA的表达与胃癌患者的肿瘤直径、淋巴结转移、浸润深度以及TNM分期有关 ,与胃癌患者的年龄、性别、分化程度及有无远处转移无关。胃癌组织中c met阳性率为 85 1% ,与肝素酶mRNA表达之间的相关系数为 0 714 (P <0 0 1)。结论 胃癌组织中肝素酶mRNA表达水平增高 ,肿瘤微血管密度增高 ,促进了胃癌的生长、浸润及转移 ,与胃癌预后不良的临床病理特征有关。c met癌基因可能参与了肝素酶的表达调控。
Objective To study the relationship between the expression of heparanase mRNA and the clinicopathological features and tumor microvessel density in gastric cancer and to explore the mechanism of increased expression of heparanase in gastric cancer. Methods The expression of heparanase mRNA was detected by reverse transcription polymerase chain reaction (RT-PCR) in 47 cases of gastric cancer tissues and adjacent normal tissues. The expression of c-met in gastric cancer tissues was detected by immunohistochemistry. The expressions of CD34 Immunohistochemical staining was used to count the tumor microvessel density. Results The positive rate of heparanase in gastric cancer tissues was significantly higher than that in corresponding adjacent tissues (74.5% vs 19.1%, P <0.01). The microvessel density of gastric cancer tissues with heparanase positive expression was significantly higher than that of negative group (51 ± 15 vs 31 ± 10, P <0.01). Heparinase mRNA expression in gastric cancer patients with tumor diameter, lymph node metastasis, depth of invasion and TNM staging, and gastric cancer patients age, gender, differentiation and distant metastasis has nothing to do. The positive rate of c-met in gastric cancer was 85 1%, and the correlation coefficient between the expression of heparin and the hepatic enzyme was 0 714 (P <0.01). Conclusions The expression of heparanase mRNA in gastric cancer tissues is increased and the microvessel density of tumor is increased, which promotes the growth, invasion and metastasis of gastric cancer, which is related to the clinicopathological features of poor prognosis of gastric cancer. c met oncogene may be involved in the regulation of heparinase expression.