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目的:建立养血止痒片中丹皮酚的HPLC含量测定方法。方法:采用Agilent C18(4.6mm×250mm,5.0μm),Column+Agilent C18(4.6mm×20mm,5.0μm),Guard Cartridge色谱柱;流动相为甲醇-水(60:40);检测波长为274nm;柱温为室温;流速为1.0mL/min。结果:丹皮酚在4.36～348.8μg/mL(r=0.999 1)范围内线性关系良好,加样回收率为97.14%,RSD为0.77%。结论:该方法简便、结果准确,可用于养血止痒片的质量控制。
Objective: To establish a HPLC method for the determination of paeonol in Yangxie Zhiyang tablets. METHODS: Guard Cartridge column was used with Agilent C18 (4.6 mm × 250 mm, 5.0 μm), Column + Agilent C18 (4.6 mm × 20 mm, 5.0 μm); the mobile phase consisted of methanol- water (60:40); the detection wavelength was 274 nm ; Column temperature was room temperature; flow rate was 1.0 mL / min. Results: Paeonol showed a good linearity in the range of 4.36 ~ 348.8 μg / mL (r = 0.999 1) with a recovery of 97.14% and a RSD of 0.77%. Conclusion: The method is simple and accurate and can be used for quality control of pruritus.