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当疟原虫的子孢子离开成熟孢子囊进入蚊唾腺时,微结构、活力、抗原性和感染性均发生改变,这些改变可能对它进一步入侵和生存有关,这可能显示了细胞膜在裂殖子对红细胞的识别和粘附的重要性。作者用自由流动电泳法对鸡疟原虫子孢子的细胞表面进行了研究:将感染有疟原虫的埃及伊蚊的中肠和唾腺,置于冷组织培养液(M199)中解剖,在组织匀浆器内磨成匀浆,经250g离心5分钟,去除蚊组织沉淀,使子孢子浮悬于M199中,在Bencham等设
As the sporozoites of Plasmodium leave the mature sporangia and enter the mosquito’s salivary glands, changes in microstructure, viability, antigenicity and infectivity are all altered, and these changes may be related to its further invasion and survival, which may indicate that the cell membrane in merozoites The importance of red blood cell recognition and adhesion. The authors used free-flow electrophoresis to study the cell surface of P. falciparum sporozoites: the midgut and salivary glands of Aedes aegypti infected with Plasmodium were placed in cold tissue culture medium (M199) and dissected to homogeneity in the tissue Slurries were homogenized and centrifuged at 250g for 5 minutes to remove mosquito tissue pellets and allow the sporozoites to float in M199. In Bencham et al.