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目的探讨不同剂量紫苏油对大鼠血脂水平和内脏脂肪含量影响。方法 40只Wistar雄性大鼠随机分为5组:对照组、高脂组及紫苏油低、中、高剂量组(6%、10%、15%),饲以相应的配方饲料8周;以酶比色法测定大鼠血清总胆固醇(TC)和甘油三酯(TG)水平,聚乙烯硫酸沉淀法测定低密度脂蛋白胆固醇(LDL-C)水平,磷钨酸-镁沉淀法测定高密度脂蛋白胆固醇(HDL-C)水平;取肝脏,分离双侧肾脏周围、附睾周围脂肪垫,分别称重。结果与对照组比较,高脂组大鼠血清TC水平[(1.90±0.37)mmol/L]升高(P<0.05);与高脂组比较,紫苏油低、中、高剂量组大鼠血清TC水平[分别为(1.54±0.36)、(1.22±0.14)、(1.34±0.22)mmol/L]均降低(P<0.05);与高脂组大鼠血清TG和HDL-C水平[分别为(0.65±0·11)、(0.77±0.12)mmol/L]比较,高剂量紫苏油组大鼠TG和HDL-C水平[分别为(0.44±0.11)、(0.56±0.14)mmol/L]均降低(P<0.05);与对照组比较,高脂组及紫苏油低、中、高剂量组大鼠肝系数均升高(P<0.05)。结论紫苏油可降低高脂大鼠血清TC水平,但当饲料中紫苏油含量过高时(15%)也会降低大鼠血清HDL-C水平。
Objective To investigate the effects of different doses of perilla oil on blood lipids and visceral fat content in rats. Methods Forty male Wistar rats were randomly divided into five groups: control, hyperlipidemia and perilla oil low, medium and high dose groups (6%, 10%, 15%), fed with the corresponding formula for 8 weeks; The levels of total cholesterol (TC) and triglyceride (TG) in rat serum were determined by enzymatic colorimetric method. The levels of low density lipoprotein cholesterol (LDL-C) were determined by polyethylene sulfate precipitation method. Density lipoprotein cholesterol (HDL-C) levels; take the liver, isolated around the kidneys, epididymal fat pad around, were weighed. Results Compared with the control group, the level of serum TC was significantly increased in the hyperlipidemic rats [(1.90 ± 0.37) mmol / L] (P <0.05). Compared with the hyperlipidemic rats, The level of serum TC and HDL-C in serum were significantly lower than those in high fat diet group [(1.54 ± 0.36) and (1.22 ± 0.14) and (1.34 ± 0.22) mmol / L, respectively (0.44 ± 0.11) and (0.56 ± 0.14) mmol / L, respectively, in the high-dose perilla oil group (0.65 ± 0.11, 0.77 ± 0.12 mmol / L] L] (P <0.05). Compared with the control group, the hepatic coefficient of high, medium and high dose group were significantly increased (P <0.05). Conclusion Perilla oil can reduce the level of serum TC in hyperlipidemic rats, but when the perilla oil content is too high (15%), the serum HDL-C level will also decrease.